Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus

STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CH...

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Published inStem cell research Vol. 34; p. 101378
Main Authors Schuster, Stefanie, Saravanakumar, Srinethe, Schöls, Ludger, Hauser, Stefan
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.01.2019
Elsevier
Online AccessGet full text
ISSN1873-5061
1876-7753
1876-7753
DOI10.1016/j.scr.2018.101378

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Summary:STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency.
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ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2018.101378