Suppression of SDP1 Improves Soybean Seed Composition by Increasing Oil and Reducing Undigestible Oligosaccharides

In developing soybean seeds, carbon is partitioned between oil, protein and carbohydrates. Here, we demonstrate that suppression of lipase-mediated turnover of triacylglycerols (TAG) during late seed development increases fatty acid content and decreases the presence of undigestible oligosaccharides...

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Published inFrontiers in plant science Vol. 13; p. 863254
Main Authors Aznar-Moreno, Jose A., Mukherjee, Thiya, Morley, Stewart A., Duressa, Dechassa, Kambhampati, Shrikaar, Chu, Kevin L., Koley, Somnath, Allen, Doug K., Durrett, Timothy P.
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 23.03.2022
Subjects
lipase activity
oil
Plant Science
protein
raffinose family oligosaccharides
SDP1
soybeans
raffinose family oligosaccharides
oil
SDP1
lipase activity
protein
soybeans
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ISSN1664-462X
1664-462X
DOI10.3389/fpls.2022.863254

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Summary:In developing soybean seeds, carbon is partitioned between oil, protein and carbohydrates. Here, we demonstrate that suppression of lipase-mediated turnover of triacylglycerols (TAG) during late seed development increases fatty acid content and decreases the presence of undigestible oligosaccharides. During late stages of embryo development, the fatty acid content of soybean seed decreases while the levels of the oligosaccharides raffinose and stachyose increase. Three soybean genes orthologous to the Arabidopsis lipase gene SUGAR-DEPENDENT1 ( SDP1 ) are upregulated at this time. Suppression of these genes resulted in higher oil levels, with lipid levels in the best lines exceeding 24% of seed weight. In addition, lipase-suppressed lines produced larger seeds compared to wild-type plants, resulting in increases of over 20% in total lipid per seed. Levels of raffinose and stachyose were lower in the transgenic lines, with average reductions of 15% in total raffinose family oligosaccharides observed. Despite the increase in oil, protein content was not negatively impacted and trended higher in the transgenic lines. These results are consistent with a role for SDP1 in turning over TAG to supply carbon for other needs, including the synthesis of oligosaccharides, and offer new strategies to further improve the composition of soybean seeds.
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Reviewed by: Changcheng Xu, Brookhaven National Laboratory (DOE), United States; Nacer Bellaloui, Crop Genetics Research Unit (USDA-ARS), United States; Peter Eastmond, Rothamsted Research, United Kingdom
Present address: Jose A. Aznar-Moreno, Department of Molecular Biology and Biochemistry, Institute of Subtropical and Mediterranean Horticulture “La Mayora”-University of Málaga-Spanish National Research Council (IHSM-UMA-CSIC), Málaga, Spain
This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science
These authors have contributed equally to this work
Edited by: Ling Li, Mississippi State University, United States
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.863254