Epitope analysis of DQ6-reactive antibodies in sera from a DQ6-positive transplant candidate sensitized during pregnancy

• Published in: Transplant immunology Vol. 38; pp. 15 - 18
• Main Authors: Daniëls, Liesbeth, Emonds, Marie-Paule, Bosmans, Jean-Louis, Marrari, Marilyn, Duquesnoy, Rene J.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2016
• Subjects: Algorithms; Alleles; Allergy and Immunology; Autoantigens - genetics; Autoantigens - immunology; Autoantigens - metabolism; Epitope; Epitope Mapping - methods; Female; High-Throughput Nucleotide Sequencing; Histocompatibility Testing; HLA; HLA antibodies; HLA-DQ Antigens - genetics; HLA-DQ Antigens - immunology; HLA-DQ Antigens - metabolism; HLAMatchmaker; Humans; Immunization; Isoantibodies - blood; Isoantigens - genetics; Isoantigens - immunology; Isoantigens - metabolism; Middle Aged; Nonself-self; Pregnancy; Protein Conformation; Registries; Transplant Recipients; Waiting Lists; HLAMatchmaker; Epitope; HLA; Nonself-self; HLA antibodies
• ISSN: 0966-3274; 1878-5492
• DOI: 10.1016/j.trim.2016.07.004

This case report describes DQ6-reactive serum antibody reactivity in a patient who types as DQ6. DNA typing showed DQB1*06:09 on the antibody producer and serum reactivity with DQB1*06:01, *06:02 and *06:03 but not with *06:04 and *06:09. HLAMatchmaker serum analysis showed antibody reactivity with a new antibody-verified 85VA eplet on DQB but additional reactivity with DQB1*02:01 could not be readily interpreted. After applying the nonself-self algorithm of HLA immunogenicity we have identified a new DQB epitope structurally described as 140A2+130R+135D and shared by DQB1*02:01 and DQB1*05:01 and DQB1*06:02 of the immunizer. •High-resolution HLA typing important to define acceptable mismatches•New DQB antibody-verified 85VA eplet analyzed by HLAMatchmaker•New DQB epitope 140A2+130R+135D analyzed by applying the nonself-self algorithm