Application of aptamers for assessment of vaccine efficacy

• Published in: Clinical and experimental vaccine research (Seoul) Vol. 6; no. 2; pp. 160 - 163
• Main Authors: Yoon, Soo Young, Gee, Grace, Hong, Kee-Jong, Seo, Sang-Hwan
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Vaccine Society 01.07.2017; The Korean Vaccine Society; 대한백신학회
• Subjects: Antibodies; Biosensors; Brief Communication; Enzymes; Immunization; Ligands; Pathogens; Quantum dots; Signal to noise ratio; Vaccines; 예방의학; Antibodies; Antigens; Vaccines; Enzyme-linked immunosorbent assay; Aptamers
• ISSN: 2287-3651; 2287-366X
• DOI: 10.7774/cevr.2017.6.2.160

Assessing antigen concentration of vaccine is essential step in determining the quality of the vaccine prior to vaccination. After vaccination, vaccine-induced antibody titer should also be measured to verify the vaccine efficacy. Since conventional assay used for vaccine concentrations and induced Ab-titers is antibody-based enzyme-linked immunosorbent assay, the assay inevitably brings drawbacks of antibody such as high cost for production, limited stability, and inconsistent quality between lot-to-lots. Aptamer is single-stranded nucleic acid having three-dimensional structure and has features overcoming limitations of antibody. This review will briefly introduce the features of aptamer and potential of aptamer-based system for evaluation of vaccine efficacy.