High-throughput profiling of posttranslational modification enzymes by phage display
Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we...
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| Published in | BioTechniques Vol. 43; no. 1; pp. 31 - 37 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
Natick, MA
Future Science Ltd
01.07.2007
Eaton Taylor & Francis Group |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0736-6205 1940-9818 |
| DOI | 10.2144/000112502 |
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| Abstract | Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidation the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification. |
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| AbstractList | Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidation the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification. Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidating the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification. Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidating the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification.Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidating the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification. |
| Author | Yen, Michelle Yin, Jun |
| AuthorAffiliation | 1Department of Chemistry, The University of Chicago, Chicago, IL, USA |
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| Keywords | High throughput screening Posttranslational modification Phage display Enzyme |
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| SubjectTerms | Acyl Carrier Protein - analysis Bacterial Proteins - metabolism Biological and medical sciences Cloning, Molecular Diverse techniques Enzymes - analysis Fundamental and applied biological sciences. Psychology Molecular and cellular biology Pantetheine - analogs & derivatives Pantetheine - chemistry Peptide Library Peptide Synthases - analysis Polyketide Synthases - analysis Protein Processing, Post-Translational Transferases (Other Substituted Phosphate Groups) - metabolism |
| Title | High-throughput profiling of posttranslational modification enzymes by phage display |
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