High-throughput profiling of posttranslational modification enzymes by phage display

Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we...

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Bibliographic Details
Published inBioTechniques Vol. 43; no. 1; pp. 31 - 37
Main Authors Yen, Michelle, Yin, Jun
Format Journal Article
LanguageEnglish
Published Natick, MA Future Science Ltd 01.07.2007
Eaton
Taylor & Francis Group
Subjects
Acyl Carrier Protein - analysis
Bacterial Proteins - metabolism
Biological and medical sciences
Cloning, Molecular
Diverse techniques
Enzymes - analysis
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Pantetheine - analogs & derivatives
Pantetheine - chemistry
Peptide Library
Peptide Synthases - analysis
Polyketide Synthases - analysis
Protein Processing, Post-Translational
Transferases (Other Substituted Phosphate Groups) - metabolism
High throughput screening
Posttranslational modification
Phage display
Enzyme
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ISSN0736-6205
1940-9818
DOI10.2144/000112502

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Summary:Phage display has been used as a high-throughput platform for identifying proteins or peptides with desired binding or catalytic activities from a complex proteome. Recently, phage display has been applied to profile the catalytic activities of posttranslational modification (PTM) enzymes. Here, we highlight recent work elucidation the downstream targets of PTM enzymes by phage display, including the genome-wide profiling of biosynthetic enzymes subject to phosphopantetheinyl transferase (PPTase) modification.
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ISSN:0736-6205
1940-9818
DOI:10.2144/000112502