Heterologous Expression of PKPI and Pin1 Proteinase Inhibitors Enhances Plant Fitness and Broad-Spectrum Resistance to Biotic Threats

• Published in: Frontiers in plant science Vol. 11; p. 461
• Main Authors: Turrà, David, Vitale, Stefania, Marra, Roberta, Woo, Sheridan L., Lorito, Matteo
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 30.04.2020
• Subjects: Alternaria alternata; Botrytis cinerea; Pin1; PKPI; plant cell proliferation; Plant Science; Pseudomonas syringae; PKPI; Botrytis cinerea; disease resistance; Pseudomonas syringae; Pin1; Alternaria alternata; plant cell proliferation
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2020.00461

Kunitz-type (PKPI) and Potato type I (Pin1) protease inhibitors (PIs) are two families of serine proteinase inhibitors often associated to plant storage organs and with well known insecticidal and nematicidal activities. Noteworthy, their ability to limit fungal and bacterial pathogenesis or to influence plant physiology has not been investigated in detail. To this aim, we generated a set of PVX-based viral constructs to transiently and heterologously express two potato ( , ) and three potato ( , , ) genes in plants, a widely used model for plant-pathogen interaction studies. Interestingly, transgenic plants expressing most of the tested PIs showed to be highly resistant against two economically important necrotrophic fungal pathogens, and . Unexpectedly, overexpression of the Kunitz-type or of the and Potato type I inhibitor genes also lead to a dramatic reduction in the propagation and symptom development produced by the bacterial pathogen . We further found that localized expression of and in leaves caused an increase in cell expansion and proliferation which lead to tissue hypertrophy and trichome accumulation. In line with this, the systemic expression of these proteins resulted in plants with enhanced shoot and root biomass. Collectively, our results indicate that PKPI and Pin1 PIs might represent valuable tools to simultaneously increase plant fitness and broad-spectrum resistance toward phytopathogens.