Multimodal Magnetic Resonance Imaging reveals alterations of sensorimotor circuits in restless legs syndrome

• Published in: Sleep (New York, N.Y.) Vol. 42; no. 12; p. 1
• Main Authors: Stefani, Ambra, Mitterling, Thomas, Heidbreder, Anna, Steiger, Ruth, Kremser, Christian, Frauscher, Birgit, Gizewski, Elke R, Poewe, Werner, Högl, Birgit, Scherfler, Christoph
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 01.12.2019
• Subjects: Adult; Aged; Anisotropy; Brain Mapping - methods; Cohort Studies; Diffusion Tensor Imaging - methods; Female; Gray Matter - diagnostic imaging; Gray Matter - physiopathology; Humans; Iron; Magnetic resonance imaging; Magnetic Resonance Imaging - methods; Male; Middle Aged; Motor Cortex - diagnostic imaging; Motor Cortex - physiopathology; Multimodal Imaging - methods; Nerve Net - diagnostic imaging; Nerve Net - physiopathology; Neurons; Restless legs syndrome; Restless Legs Syndrome - diagnostic imaging; Restless Legs Syndrome - physiopathology; White Matter - diagnostic imaging; White Matter - physiopathology; Young Adult; Austria; brain imaging; transverse relaxation rate; restless legs syndrome; RLS; diffusion tensor imaging; iron; voxel-based morphometry; neuroimaging
• ISSN: 0161-8105; 1550-9109; 1550-9109
• DOI: 10.1093/sleep/zsz171

Abstract Study Objectives Integrated information on brain microstructural integrity and iron storage and its impact on the morphometric profile is not available in restless legs syndrome (RLS). We applied multimodal magnetic resonance imaging (MRI) including diffusion tensor imaging, the transverse relaxation rate (R2*), a marker for iron storage, as well as gray and white matter volume measures to characterize RLS-related MRI signal distribution patterns and to analyze their associations with clinical parameters. Methods Eighty-seven patients with RLS (mean age 51, range 20–72 years; disease duration, mean 13 years, range 1–46 years, of those untreated n = 30) and 87 healthy control subjects, individually matched for age and gender, were investigated with multimodal 3T MRI. Results Volume of the white matter compartment adjacent to the post- and precentral cortex and fractional anisotropy (FA) of the frontopontine tract were both significantly reduced in RLS compared to healthy controls, and these alterations were associated with disease duration (r = 0.25, p = 0.025 and r = 0.23, p = 0.037, respectively). Corresponding gray matter volume increases of the right primary motor cortex in RLS (p < 0.001) were negatively correlated with the right FA signal of the frontopontine tract (r = −0.22; p < 0.05). Iron content evaluated with R2* was reduced in the putamen as well as in temporal and occipital compartments of the RLS cohort compared to the control group (p < 0.01). Conclusions Multimodal MRI identified progressing white matter decline of key somatosensory circuits that may underlie the perception of sensory leg discomfort. Increases of gray matter volume of the premotor cortex are likely to be a consequence of functional neuronal reorganization.