Determination of benzimidazole residues in bovine milk by ultra-high performance liquid chromatography–tandem mass spectrometry

A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC–MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in...

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Published in	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 878; no. 30; pp. 3174 - 3180
Main Authors	Xia, Xi, Dong, Yichun, Luo, Pengjie, Wang, Xia, Li, Xiaowei, Ding, Shuangyang, Shen, Jianzhong
Format	Journal Article
Language	English
Published	Amsterdam Elsevier B.V 15.11.2010 Elsevier
Subjects	Analysis Analytical, structural and metabolic biochemistry Animals Anthelmintics - analysis Benzimidazole Benzimidazoles - analysis Biological and medical sciences Calibration Cartridges Cattle Chromatography Chromatography, High Pressure Liquid - methods Drug Residues - analysis Extraction Fundamental and applied biological sciences. Psychology General pharmacology Liquids Mass spectrometry Medical sciences Milk Milk - chemistry Pharmacology. Drug treatments Residues Tandem mass spectrometry Tandem Mass Spectrometry - methods Ultra-high performance liquid chromatography Tandem mass spectrometry Benzimidazole Ultra-high performance liquid chromatography Milk Ultra performance liquid chromatography Benzimidazole derivatives Residue Mass spectrometry MS/MS HPLC chromatography Cow milk Determination Quantitative analysis
Online Access	Get full text
ISSN	1570-0232 1873-376X 1873-376X
DOI	10.1016/j.jchromb.2010.09.026

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Abstract	A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC–MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L −1 and from 0.1 to 1.0 μg L −1, respectively.
AbstractList	A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC–MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L −1 and from 0.1 to 1.0 μg L −1, respectively. A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L⁻¹ and from 0.1 to 1.0 μg L⁻¹, respectively. A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 kg L super(-1) and from 0.1 to 1.0 kg L super(-1), respectively. A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L⁻¹ and from 0.1 to 1.0 μg L⁻¹, respectively.A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L⁻¹ and from 0.1 to 1.0 μg L⁻¹, respectively.
Author	Luo, Pengjie Ding, Shuangyang Li, Xiaowei Shen, Jianzhong Dong, Yichun Xia, Xi Wang, Xia
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Keywords	Tandem mass spectrometry Benzimidazole Ultra-high performance liquid chromatography Milk Ultra performance liquid chromatography Benzimidazole derivatives Residue Mass spectrometry MS/MS HPLC chromatography Cow milk Determination Quantitative analysis
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Snippet	A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC–MS/MS) for the simultaneous... A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC-MS/MS) for the simultaneous...
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SubjectTerms	Analysis Analytical, structural and metabolic biochemistry Animals Anthelmintics - analysis Benzimidazole Benzimidazoles - analysis Biological and medical sciences Calibration Cartridges Cattle Chromatography Chromatography, High Pressure Liquid - methods Drug Residues - analysis Extraction Fundamental and applied biological sciences. Psychology General pharmacology Liquids Mass spectrometry Medical sciences Milk Milk - chemistry Pharmacology. Drug treatments Residues Tandem mass spectrometry Tandem Mass Spectrometry - methods Ultra-high performance liquid chromatography
Title	Determination of benzimidazole residues in bovine milk by ultra-high performance liquid chromatography–tandem mass spectrometry
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