Determination of benzimidazole residues in bovine milk by ultra-high performance liquid chromatography–tandem mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 878; no. 30; pp. 3174 - 3180
• Main Authors: Xia, Xi, Dong, Yichun, Luo, Pengjie, Wang, Xia, Li, Xiaowei, Ding, Shuangyang, Shen, Jianzhong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.11.2010; Elsevier
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Anthelmintics - analysis; Benzimidazole; Benzimidazoles - analysis; Biological and medical sciences; Calibration; Cartridges; Cattle; Chromatography; Chromatography, High Pressure Liquid - methods; Drug Residues - analysis; Extraction; Fundamental and applied biological sciences. Psychology; General pharmacology; Liquids; Mass spectrometry; Medical sciences; Milk; Milk - chemistry; Pharmacology. Drug treatments; Residues; Tandem mass spectrometry; Tandem Mass Spectrometry - methods; Ultra-high performance liquid chromatography; Tandem mass spectrometry; Benzimidazole; Ultra-high performance liquid chromatography; Milk; Ultra performance liquid chromatography; Benzimidazole derivatives; Residue; Mass spectrometry MS/MS; HPLC chromatography; Cow milk; Determination; Quantitative analysis
• ISSN: 1570-0232; 1873-376X; 1873-376X
• DOI: 10.1016/j.jchromb.2010.09.026

A method based on ultra-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UHPLC–MS/MS) for the simultaneous determination of benzimidazole residues in bovine milk has been optimized and validated. Rapid chromatographic separation of 13 analytes in 8 min was obtained by means of UHPLC. The samples were subject to Oasis MCX solid-phase extraction cartridges for extraction and clean-up. Matrix-matched calibration curves were performed to compensate for the matrix effect and loss in sample preparation. Mean recoveries ranged from 80% to 101% and inter-day precision was lower than 14%. Limit of detection and limit of quantification of the method ranged from 0.01 to 0.5 μg L −1 and from 0.1 to 1.0 μg L −1, respectively.