Pharmacokinetic profiles of 3-(4-hydroxy-3-methoxyphenyl) propionic acid and its conjugates in Sprague-Dawley rats

• Published in: Bioscience, biotechnology, and biochemistry Vol. 87; no. 5; pp. 516 - 524
• Main Authors: Abe, Chizumi, Soma, Ayano, Tun, Tint Ni Ni, Zhang, Ye, Nishitani, Yosuke, Kayaki, Hiroyuki, Kawakami, Hideaki, Matsui, Toshiro
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 24.04.2023
• Subjects: Animals; Hempa - metabolism; Liver - metabolism; Propionates; Rats; Rats, Sprague-Dawley; metabolite; 3-(4-hydroxy-3-methoxyphenyl)propionic acid; bioavailability; absorption; tissue distribution
• ISSN: 1347-6947; 1347-6947
• DOI: 10.1093/bbb/zbad023

ABSTRACT 3-(4-hydroxy-3-methoxyphenyl)propionic acid (HMPA) is one of the end-products from gut microbiota from dietary polyphenols, which might contribute to their health benefits. This study aims to investigate the absorption, metabolism, and tissue accumulation of HMPA in Sprague-Dawley (SD) rats. After HMPA (10 mg/kg body weight) was orally administered, intact and conjugated HMPAs in the bloodstream were detected and reached the maximum concentration in 15 min (HMPA, 2.6 ± 0.4 nmol/mL; sulfated HMPA, 3.6 ± 0.9 nmol/mL; glucuronidated HMPA, 0.55 ± 0.09 nmol/mL). HMPA and its conjugates were also detected in the target organs 6 h postadministration, indicating that HMPA undergoes rapid conversion into conjugates, and they broadly distribute to organs with similar profiles (kidneys > liver > thoracic aorta > heart > soleus muscle > lungs). This study demonstrated that orally administered HMPA (10 mg/kg) in SD rats undergoes rapid metabolism and wide tissue distribution with ≥1.2% absorption ratio. Graphical Abstract Graphical Abstract The pharmacokinetic profile of 3-(4-hydroxy-3-methoxyphenyl)propionic acid, an end-product by gut microbiota derived from biofunctional polyphenols, is quantitatively clarified in SD rats with LC–TOF/MS system.