The characteristics and enzyme activities of 4-chlorophenol biodegradation by Fusarium sp
The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacit...
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Published in | Bioresource technology Vol. 102; no. 3; pp. 2985 - 2989 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
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01.02.2011
[New York, NY]: Elsevier Ltd Elsevier |
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ISSN | 0960-8524 1873-2976 1873-2976 |
DOI | 10.1016/j.biortech.2010.10.006 |
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Abstract | The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacity on degrading 4-CP in solution. The optimum values of pH, sucrose concentration and temperature are pH 7,1g/L and 30°C, respectively. The strain can produce chlorocatechol 1,2-dioxygenase (CC12O) and chlorocatechol 2,3-dioxygenase (CC23O), which show the highest activities when 4-CP is used as the sole carbon source and energy, and the optimal values of pH and temperature are pH 7 and 50°C for CC12O as well as pH 8 and 60°C for CC23O. The kinetics of enzyme-catalyzed reactions accord with the Michaelis–Menten equation. To our knowledge, this is the first study on biodegradation of 4-CP by Fusarium sp. HJ01. |
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AbstractList | The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacity on degrading 4-CP in solution. The optimum values of pH, sucrose concentration and temperature are pH 7,1 g/L and 30°C, respectively. The strain can produce chlorocatechol 1,2-dioxygenase (CC12O) and chlorocatechol 2,3-dioxygenase (CC23O), which show the highest activities when 4-CP is used as the sole carbon source and energy, and the optimal values of pH and temperature are pH 7 and 50°C for CC12O as well as pH 8 and 60°C for CC23O. The kinetics of enzyme-catalyzed reactions accord with the Michaelis-Menten equation. To our knowledge, this is the first study on biodegradation of 4-CP by Fusarium sp. HJ01.The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacity on degrading 4-CP in solution. The optimum values of pH, sucrose concentration and temperature are pH 7,1 g/L and 30°C, respectively. The strain can produce chlorocatechol 1,2-dioxygenase (CC12O) and chlorocatechol 2,3-dioxygenase (CC23O), which show the highest activities when 4-CP is used as the sole carbon source and energy, and the optimal values of pH and temperature are pH 7 and 50°C for CC12O as well as pH 8 and 60°C for CC23O. The kinetics of enzyme-catalyzed reactions accord with the Michaelis-Menten equation. To our knowledge, this is the first study on biodegradation of 4-CP by Fusarium sp. HJ01. The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacity on degrading 4-CP in solution. The optimum values of pH, sucrose concentration and temperature are pH 7,1g/L and 30°C, respectively. The strain can produce chlorocatechol 1,2-dioxygenase (CC12O) and chlorocatechol 2,3-dioxygenase (CC23O), which show the highest activities when 4-CP is used as the sole carbon source and energy, and the optimal values of pH and temperature are pH 7 and 50°C for CC12O as well as pH 8 and 60°C for CC23O. The kinetics of enzyme-catalyzed reactions accord with the Michaelis-Menten equation. To our knowledge, this is the first study on biodegradation of 4-CP by Fusarium sp. HJ01. The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacity on degrading 4-CP in solution. The optimum values of pH, sucrose concentration and temperature are pH 7,1 g/L and 30°C, respectively. The strain can produce chlorocatechol 1,2-dioxygenase (CC12O) and chlorocatechol 2,3-dioxygenase (CC23O), which show the highest activities when 4-CP is used as the sole carbon source and energy, and the optimal values of pH and temperature are pH 7 and 50°C for CC12O as well as pH 8 and 60°C for CC23O. The kinetics of enzyme-catalyzed reactions accord with the Michaelis-Menten equation. To our knowledge, this is the first study on biodegradation of 4-CP by Fusarium sp. HJ01. |
Author | Li, Jiwu Cai, Weijian Zhu, Ling |
Author_xml | – sequence: 1 givenname: Jiwu surname: Li fullname: Li, Jiwu email: li169@sina.com – sequence: 2 givenname: Weijian surname: Cai fullname: Cai, Weijian – sequence: 3 givenname: Ling surname: Zhu fullname: Zhu, Ling |
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Keywords | Chlorocatechol 2,3-dioxygenase 4-Chlorophenol biodegradation Fusarium sp. HJ01 Chlorocatechol 1,2-dioxygenase Biodegradation HJ01 Enzyme Fusarium sp Fungi Chlorophenol Enzymatic activity Fusarium Fungi Imperfecti Oxidoreductases |
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SubjectTerms | 4-Chlorophenol biodegradation biodegradation Biodegradation, Environmental Biological and medical sciences Biotechnology Catechol 1,2-Dioxygenase Catechol 1,2-Dioxygenase - metabolism Chlorocatechol 1,2-dioxygenase Chlorocatechol 2,3-dioxygenase chlorophenols Chlorophenols - metabolism classification Dioxygenases Dioxygenases - metabolism Enzyme Activation enzyme activity enzyme kinetics equations Fundamental and applied biological sciences. Psychology Fusarium Fusarium - classification Fusarium - metabolism Fusarium sp. HJ01 metabolism oxygenases Species Specificity sucrose temperature |
Title | The characteristics and enzyme activities of 4-chlorophenol biodegradation by Fusarium sp |
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