The characteristics and enzyme activities of 4-chlorophenol biodegradation by Fusarium sp

• Published in: Bioresource technology Vol. 102; no. 3; pp. 2985 - 2989
• Main Authors: Li, Jiwu, Cai, Weijian, Zhu, Ling
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2011; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: 4-Chlorophenol biodegradation; biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; Catechol 1,2-Dioxygenase; Catechol 1,2-Dioxygenase - metabolism; Chlorocatechol 1,2-dioxygenase; Chlorocatechol 2,3-dioxygenase; chlorophenols; Chlorophenols - metabolism; classification; Dioxygenases; Dioxygenases - metabolism; Enzyme Activation; enzyme activity; enzyme kinetics; equations; Fundamental and applied biological sciences. Psychology; Fusarium; Fusarium - classification; Fusarium - metabolism; Fusarium sp. HJ01; metabolism; oxygenases; Species Specificity; sucrose; temperature; Chlorocatechol 2,3-dioxygenase; 4-Chlorophenol biodegradation; Fusarium sp. HJ01; Chlorocatechol 1,2-dioxygenase; Biodegradation; HJ01; Enzyme; Fusarium sp; Fungi; Chlorophenol; Enzymatic activity; Fusarium; Fungi Imperfecti; Oxidoreductases
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2010.10.006

The effects of pH, temperature and sucrose addition on biodegradative capacity of Fusarium sp. HJ01 for 4-chlorophenol (4-CP) were examined, the property of dioxygenases produced by Fusarium sp. HJ01 during 4-CP degradation was investigated. The results show that Fusarium sp. HJ01 has a high capacity on degrading 4-CP in solution. The optimum values of pH, sucrose concentration and temperature are pH 7,1g/L and 30°C, respectively. The strain can produce chlorocatechol 1,2-dioxygenase (CC12O) and chlorocatechol 2,3-dioxygenase (CC23O), which show the highest activities when 4-CP is used as the sole carbon source and energy, and the optimal values of pH and temperature are pH 7 and 50°C for CC12O as well as pH 8 and 60°C for CC23O. The kinetics of enzyme-catalyzed reactions accord with the Michaelis–Menten equation. To our knowledge, this is the first study on biodegradation of 4-CP by Fusarium sp. HJ01.