Rapid and sensitive biosensor for Salmonella

• Published in: Biosensors & bioelectronics Vol. 15; no. 3; pp. 135 - 141
• Main Authors: Pathirana, S.T, Barbaree, J, Chin, B.A, Hartell, M.G, Neely, W.C, Vodyanoy, V
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.06.2000; Elsevier Science
• Subjects: Acoustic wave device; Animals; Antibodies, Bacterial; Antigen–antibody monolayers; Bacteriological Techniques; Biological and medical sciences; Biosensing Techniques - methods; Biosensing Techniques - statistics & numerical data; Biosensors; Biotechnology; Food Microbiology; Fundamental and applied biological sciences. Psychology; Humans; Langmuir–Blodgett immobilization; Methods. Procedures. Technologies; Salmonella typhimurium; Salmonella typhimurium - immunology; Salmonella typhimurium - isolation & purification; Sensitivity and Specificity; Sensor; Time Factors; Various methods and equipments; Acoustic wave device; Antigen–antibody monolayers; Langmuir–Blodgett immobilization; Sensor; Performance evaluation; Monolayer; Antibody; Biosensor; Immobilization; Bacteria; Quartz; Salmonella typhimurium; Immunosensor; Enterobacteriaceae; Langmuir Blodgett method
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(00)00067-1

The rapid and sensitive detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir–Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated. The binding of bacteria to the surface changed the crystal resonance parameters; these were quantified by the output voltage of the sensor instrumentation. The sensor had a lower detection limit of a few hundred cells/ml, and a response time of <100 s over the range of 10 2–10 10 cells/ml. The sensor response was linear between bacterial concentrations of 10 2–10 7 cells/ml, with a sensitivity of 18 mV/decade. The binding of bacteria was specific with two binding sites needed to bind a single cell. The sensors preserve ∼75% of their sensitivity over a period of 32 days.