Proton magnetic resonance spectroscopy of skeletal muscle: A comparison of two quantitation techniques

• Published in: Journal of magnetic resonance (1997) Vol. 243; pp. 81 - 84
• Main Authors: Wang, Xin, Salibi, Nouha, Fayad, Laura M., Barker, Peter B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2014
• Subjects: Adult; Algorithms; Aspartic Acid - analogs & derivatives; Aspartic Acid - metabolism; Computer Simulation; Creatine - metabolism; Female; Humans; Male; Methylamines - metabolism; Models, Biological; MR spectroscopy; Muscle; Phantom replacement; Proton Magnetic Resonance Spectroscopy - methods; Quantitation; Reproducibility of Results; Sensitivity and Specificity; Muscle; Quantitation; Phantom replacement; MR spectroscopy
• ISSN: 1090-7807; 1096-0856; 1096-0856
• DOI: 10.1016/j.jmr.2014.03.010

[Display omitted] •Two MRS quantitation methods were compared in healthy human muscle at 3T in vivo.•They are based on phantom replacement and internal water reference respectively.•Both methods measured comparable concentrations for muscle creatine and TMA.•In healthy muscle the water reference method showed slightly lower variability.•In pathological conditions phantom replacement method may be more appropriate. The aim of this study was to develop and compare two methods for quantification of metabolite concentrations in human skeletal muscle using phased-array receiver coils at 3T. Water suppressed and un-suppressed spectra were recorded from the quadriceps muscle (vastus medialis) in 8 healthy adult volunteers, and from a calibration phantom containing 69mM/L N-acetyl aspartate. Using the phantom replacement technique, trimethylamine specifically [TMA] and creatine [Cr] concentrations were estimated, and compared to those values obtained by using the water reference method. Quadriceps [TMA] concentrations were 9.5±2.4 and 9.6±4.1mmol/kg wet weight using the phantom replacement and water referencing methods respectively, while [Cr] concentrations were 26.8±12.2 and 24.1±5.3mmol/kg wet weight respectively. Reasonable agreement between water referencing and phantom replacement methods was found, although for [Cr] variation was significantly higher for the phantom replacement technique. The relative advantages and disadvantages of each approach are discussed.