Beneficial effects of the mixed adjuvant of CpG plus monophosphoryl lipid a in immunization with a recombinant protein vaccine for hepatitis A

• Published in: Applied biological chemistry Vol. 56; no. 1; pp. 95 - 98
• Main Authors: Hwang, Inkyu, Choi, Daewoon, See, Hyejeong, Kim, Wonyong, Chung, In Sik, Shon, Dong-Hwa
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.02.2013; Springer Nature B.V; 한국응용생명화학회
• Subjects: Adjuvants; alum; Antigens; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; CpG islands; Cytokines; Food contamination; Hepatitis; Hepatitis A; Hepatitis A virus; Hepatovirus A; Immunization; Immunoglobulin G; Immunology; Lipid A; Lipids; Lymphocytes T; Monophosphoryl lipid A; oligodeoxyribonucleotides; Proteins; recombinant proteins; Short Communication; vaccine development; Vaccines; Viruses; VP1 protein; 농학; cytokine; recombinant vaccine; hepatitis A; immunoglobulin; adjuvant
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2234-344X; 2468-0842
• DOI: 10.1007/s13765-012-2398-5

In an effort to develop a new vaccine for hepatitis A, which is mainly transmitted via contaminated foods and water, recombinant virus protein 1 (VP1) of hepatitis A virus was used as an antigen. Several adjuvants in a single or a mixed form, i.e., alum, CpG oligodeoxynucleotide, monophosphoryl lipid A (MPL), alum plus MPL, and CpG plus MPL, were also tested for their immunological properties. When their effects on the production of VP1-specific IgG relative to that of total IgG and the levels of and balance between Th1- and Th2-type cytokine productions were compared, CpG plus MPL was found to have highly beneficial effects, providing a new insight in selection of adjuvant for development of a new vaccine.