Characterization of IL-17AA and IL-17FF in rheumatoid arthritis and multiple sclerosis

• Published in: Bioanalysis Vol. 8; no. 22; pp. 2317 - 2327
• Main Authors: Schofield, Cheryl, Fischer, Saloumeh K, Townsend, Michael J, Mosesova, Sofia, Peng, Kun, Setiadi, Alvernia Francesca, Song, An, Baruch, Amos
• Format: Journal Article
• Language: English
• Published: England Future Science Ltd 01.11.2016
• Subjects: Arthritis, Rheumatoid - blood; Arthritis, Rheumatoid - cerebrospinal fluid; CSF; Humans; IL-17A; IL-17F; Immunoassay - methods; Interleukin-17 - blood; Interleukin-17 - cerebrospinal fluid; Limit of Detection; multiple sclerosis; Multiple Sclerosis - blood; Multiple Sclerosis - cerebrospinal fluid; Recurrence; rheumatoid arthritis; serum; IL-17F; IL-17A; serum; rheumatoid arthritis; multiple sclerosis; CSF
• ISSN: 1757-6180; 1757-6199; 1757-6199
• DOI: 10.4155/bio-2016-0207

IL-17 is thought to play a prominent role in immune disorders. Sensitive and specific IL-17AA and IL-17FF assays were developed and used to determine levels in serum and cerebrospinal fluid (CSF) from patients with rheumatoid arthritis and relapsing remitting multiple sclerosis (RRMS). Qualified assays detected IL-17AA and IL-17FF in healthy and disease samples. Serum IL-17AA was significantly higher in rheumatoid arthritis and RRMS as compared with normal healthy subjects. IL-17AA was also elevated in RRMS CSF as compared with normal healthy subjects; although correlation was observed between serum levels of the two isoforms, no correlation was detected between serum and CSF levels. Reliable determination of IL-17 isoforms in the systemic and CNS compartments sheds light on the involvement of IL-17AA and IL-17FF in autoimmunity.