Retinoic Acid-Induced Gene G(RIG-G) as a Novel Monitoring Biomarker in Leukemia and Its Clinical Applications

• Published in: Genes Vol. 12; no. 7; p. 1035
• Main Authors: Wang, Fei, Tian, Jiale, Pang, Li, Wu, Junlu, Shang, Anquan, Sun, Zujun, Li, Dong, Yan, Jinsong, Quan, Wenqiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.07.2021; MDPI
• Subjects: Acids; Acute myeloid leukemia; Acute promyeloid leukemia; Adolescent; Adult; Aged; Aged, 80 and over; Biomarkers; Blood; Bone Marrow; Cell cycle; Female; fluorescent dyes; Fluorescent indicators; Gene expression; Gene Expression Regulation - drug effects; genes; Humans; Intracellular Signaling Peptides and Proteins - genetics; Laboratories; Leukemia; Leukemia, Promyelocytic, Acute - blood; Leukemia, Promyelocytic, Acute - drug therapy; Leukemia, Promyelocytic, Acute - genetics; Male; Medical prognosis; Middle Aged; Patients; Peripheral blood; Polymerase chain reaction; Promyeloid leukemia; quantitative polymerase chain reaction; Remission; Retinoic acid; RNA, Messenger - metabolism; Software; Tretinoin - pharmacology; Young Adult; United States > US; San Francisco California; China; RIG-G gene; acute promyelocytic leukemia; real-time polymerase chain reaction
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes12071035

Retinoic acid inducible gene G (RIG-G) is an inducible gene produced during the treatment of acute promyelocytic leukemia with all-trans retinoic acid (ATRA). However, it is unclear the expression level of RIG-G gene in the peripheral blood of healthy subjects and patients with acute promyelocytic leukemia (APL or AML-M3). In the present study, we established the TaqMan-MGB fluorescent probe qPCR (real-time polymerase chain reaction) method for the first time to detect the expression of RIG-G gene in APL. Twenty APL patients were selected, and their RIG-G expression levels were quantified to assess the correlation between the expression of peripheral blood and bone marrow samples. U test was used to analyze the expression level of RIG-G in the peripheral blood of 40 normal specimens and 20 APL patients to observe the prognostic monitoring effect of RIG-G gene in the ATRA treatment process. ROC (receiver operating characteristic curve) was used to analyze and test the diagnostic efficiency of RIG-G gene for APL patients. There is a strong positive correlation between the expression of RIG-G in peripheral blood and bone marrow of APL patients. The expression level of RIG-G in peripheral blood of APL patients is significantly lower than that in healthy controls (p < 0.001). The changes in the expression level of RIG-G in peripheral blood changed indicates the remission and recurrence of APL patients after ATRA treatment, and the ROC curve shows that it has a better diagnostic power for APL. In summary, the TaqMan-MGB real-time PCR method we have established has successfully run. The detection of RIG-G gene expression in peripheral blood can effectively monitor the disease changes of APL patients and avoid harmful bone marrow puncture injury.