Serum transfer RNA‐derived fragment tRF‐31‐79MP9P9NH57SD acts as a novel diagnostic biomarker for non‐small cell lung cancer

• Published in: Journal of clinical laboratory analysis Vol. 36; no. 7
• Main Authors: Li, Jipeng, Cao, Chao, Fang, Laifu, Yu, Wanjun
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.07.2022; John Wiley and Sons Inc
• Subjects: biomarker; Biomarkers; Cell growth; Human subjects; Investigations; Lung cancer; Lymph nodes; Lymphatic system; Malignancy; Non-small cell lung carcinoma; NSCLC; Patients; Small cell lung carcinoma; Statistical analysis; Transfer RNA; tRF‐31‐79MP9P9NH57SD; tRNA; tRNA‐derived small RNAs; NSCLC; biomarker; tRF-31-79MP9P9NH57SD; tRNA-derived small RNAs
• ISSN: 0887-8013; 1098-2825; 1098-2825
• DOI: 10.1002/jcla.24492

Background tRNA‐derived fragments (tRFs) have been found to have a crucial function in the pathophysiology of cancers. However, the function of tRFs in non‐small cell lung cancer (NSCLC) is yet unknown. The goal of this study was to assess the tRF‐31‐79MP9P9NH57SD serum expression from NSCLC patients and to determine its diagnostic usefulness. Methods By using stem‐loop quantitative real‐time PCR, we were able to detect various tRF‐31‐79MP9P9NH57SD expressions in 96 NSCLC serum samples, 96 healthy controls, and 20 pairs of NSCLC serum samples pre‐ and post‐surgery (qRT‐PCR). After that, we analyzed its diagnostic effectiveness using the receiver operating characteristic (ROC) curve. Results Serum tRF‐31‐79MP9P9NH57SD expression was higher in NSCLC patients, and levels of tRF‐31‐79MP9P9NH57SD were linked to the clinical stage (p = 0.002) and the malignancy of lymph node (p = 0.012). In addition, after the procedure, the serum tRF‐31‐79MP9P9NH57SD expression in NSCLC patients dropped. With 48.96 percent sensitivity and 90.62 percent specificity, the area under ROC curve (AUC) was 0.733. Conclusion serum tRF‐31‐79MP9P9NH57SD possibly is a new and groundbreaking biomarker for the NSCLC. Serum levels of tRF‐31‐79MP9P9NH57SD were detected in NSCLC by stem‐loop qRT‐PCR. Then clinical implications and downstream targets of tRF‐31‐79MP9P9NH57SD were analyzed.