Conserved nucleosome positioning defines replication origins

The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC bi...

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Bibliographic Details
Published inGenes & development Vol. 24; no. 8; pp. 748 - 753
Main Authors Eaton, Matthew L., Galani, Kyriaki, Kang, Sukhyun, Bell, Stephen P., MacAlpine, David M.
Format Journal Article
LanguageEnglish
Published United States Cold Spring Harbor Laboratory Press 15.04.2010
Subjects
Chromosomes, Fungal - genetics
Genome, Fungal - genetics
Nucleosomes - genetics
Replication Origin - genetics
Research Communication
Saccharomyces cerevisiae - genetics
Sequence Analysis
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ISSN0890-9369
1549-5477
1549-5477
DOI10.1101/gad.1913210

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Summary:The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.
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These authors contributed equally to this work.
ISSN:0890-9369
1549-5477
1549-5477
DOI:10.1101/gad.1913210