Gastrointestinal stability and bioavailability of (poly)phenolic compounds following ingestion of Concord grape juice by humans

• Published in: Molecular nutrition & food research Vol. 56; no. 3; pp. 497 - 509
• Main Authors: Stalmach, Angelique, Edwards, Christine A., Wightman, JoLynne D., Crozier, Alan
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.03.2012; WILEY‐VCH Verlag; Wiley
• Subjects: (Poly)phenol bioavailability; absorption; acids; Adult; analysis; anthocyanins; Anthocyanins - blood; Anthocyanins - urine; Beverages; Beverages - analysis; bioavailability; Biological and medical sciences; blood; chemistry; Chromatography, High Pressure Liquid; colon; Colon - drug effects; Colon - metabolism; Concord grape juice; Digestion; drug effects; esters; excretion; Feeding. Feeding behavior; Female; Food industries; Fruit; Fruit - chemistry; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; Gastrointestinal stability; grape juice; Humans; hydrogenation; Ileum; Ileum - drug effects; Ileum - metabolism; ingestion; Male; metabolism; metabolites; microorganisms; Models, Biological; pharmacokinetics; phenolic compounds; Plasma pharmacokinetics; Polyphenols; Polyphenols - pharmacokinetics; small intestine; Urinary excretion; urine; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vitis; Vitis - chemistry; Young Adult; Human; Excretion; Stability; Fruit; Concord grape juice; Digestive system; Bioavailability; Gastrointestinal; (Poly)phenol bioavailability; Urinary excretion; Phenol; Gastrointestinal stability; Quality; Grape; Phenols; Pharmacokinetics; Plasma pharmacokinetics
• ISSN: 1613-4125; 1613-4133; 1613-4133
• DOI: 10.1002/mnfr.201100566

The in vitro gastrointestinal stability of (poly)phenolic compounds in Concord grape juice was compared with recoveries in ileal fluid after the ingestion of the juice by ileostomists. Recoveries in ileal fluid indicated that 67% of hydroxycinnamate tartarate esters, and smaller percentages of the intake of other (poly)phenolic compounds, pass from the small intestine to the colon. The juice was also ingested by healthy subjects with an intact functioning colon. Peak plasma concentrations (Cmax) ranged from 1.0 nmol/L for petunidin‐3‐O‐glucoside to 355 nmol/L for dihydrocoumaric acid. Urinary excretion, as an indicator of bioavailability, varied from 0.26% for total anthocyanins to 24% for metabolites of hydroxycinnamate tartarate esters. The Cmax times of the anthocyanins indicated that their low level absorption occurred in the small intestine in contrast to hydroxycinnamate metabolites which were absorbed in both the small and the large intestine where the colonic microflora appeared responsible for hydrogenation of the hydroxycinnamate side chain. The bioavailability of the complex mixture of (poly)phenolic compounds in Concord grape juice, was very similar to that observed in previous studies when compounds were either fed individually or as major components in products containing a restricted spectrum of (poly)phenolic compounds.