Green tea extract rich in epigallocatechin gallate impairs alveolar bone loss in ovariectomized rats with experimental periodontal disease

• Published in: International journal of experimental pathology Vol. 101; no. 6; pp. 277 - 288
• Main Authors: Vargas‐Sanchez, Paula Katherine, Pitol, Dimitrius Leonardo, Sousa, Luiz Gustavo, Beloti, Márcio Mateus, Rosa, Adalberto Luiz, Rossi, Ana Cláudia, Siéssere, Selma, Bombonato‐Prado, Karina Fittipaldi
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.12.2020; John Wiley and Sons Inc
• Subjects: Alveolar bone; animal model; Animals; Antioxidants - pharmacology; Biomedical materials; Bone density; Bone Density - drug effects; Bone loss; Bone mass; Bone resorption; Bone turnover; Cancellous bone; Catechin - analogs & derivatives; Disease; Epigallocatechin gallate; Euthanasia; experimental periodontal disease; Female; Femur; Flavonoids; Green tea; green tea extract; Mandible; Medical treatment; Metabolism; Morphometry; Original; Osteoporosis; Osteoporosis - pathology; Ovariectomy; Periodontal disease; Periodontal diseases; Periodontal Diseases - drug therapy; Periodontal Diseases - pathology; Plant Extracts - pharmacology; Rats; Rats, Wistar; Septum; Statistical methods; Surgery; Tea; experimental periodontal disease; animal model; osteoporosis; green tea extract
• ISSN: 0959-9673; 1365-2613; 1365-2613
• DOI: 10.1111/iep.12379

Periodontal disease and osteoporosis are characterized by bone resorption, and researchers have shown an association between these two diseases through increasing loss of systemic bone mass and triggering alveolar bone loss. Green tea is a common and easily accessible beverage, and evidences show that flavonoid epigallocatechin gallate (EGCG) could decrease bone loss in pathologies such as osteoporosis and periodontal disease. In order to verify its possible effects and apply them in the treatment and prevention of these diseases, this investigation aimed to evaluate the influence of green tea extract (GTE) on bone metabolism of ovariectomized rats after experimental periodontal disease (EPD) by histological, morphological and microtomographic parameters. Wistar female rats were divided into Sham, Sham + EPD, Sham + EPD + GTE, OVX, OVX + EPD and OVX + EPD + GTE groups. Immediately after surgery, gavage administration of 50 mg/kg of green tea extract (GTE) was performed for 60 days, with subsequent induction of periodontal disease by ligature 15 days before euthanasia. Mandible and femur samples were collected for histological, morphometric and microtomographic analysis. The results were analysed by means of statistical software with significance set at 5%. Histological and morphometric analysis showed a significant decrease in alveolar and femoral trabecular bone loss in groups that received GTE. Microtomographic results showed that trabecular thickness and bone surface density values in alveolar bone interradicular septum of the OVX + EPD + GTE groups were similar to the Sham group. The results obtained suggest that green tea extract may improve bone metabolism in osteoporotic rats with periodontal disease.