Antioxidant Activities of Crude Phlorotannins from Sargassum hemiphyllum

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 36; no. 3; pp. 449 - 455
• Main Author: 赵智丽 杨小青 龚忠勤 潘明珠 韩雅莉 刘义
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.06.2016; People's Hospital of Longhua District, Shenzhen 518000, China%School of Pharmacy, Guangdong Medical College, Zhanjiang 524023,China%School of Pharmacy, Guangdong Medical College, Zhanjiang 524023,China; Shenzhen Enhance Technology Co., Ltd., Shenzhen 518000, China%Department of Food and Biology, Guangdong University of Technology, Gruangzhou 510006, China%Key Laboratory of Research & Development of Nature Drug, Guangdong Medical College, Zhanjiang 524023,China; Department of Food and Biology, Guangdong University of Technology, Gruangzhou 510006, China
• Subjects: Animals; Antioxidants - isolation & purification; Antioxidants - pharmacology; Ascorbic Acid - pharmacology; Brain - drug effects; Brain - metabolism; Brain - pathology; Carbon Tetrachloride - antagonists & inhibitors; Carbon Tetrachloride - toxicity; Carbon Tetrachloride Poisoning - drug therapy; Carbon Tetrachloride Poisoning - metabolism; Carbon Tetrachloride Poisoning - pathology; CCl4; Chemical Fractionation - methods; Gallic Acid - pharmacology; Hydroxyl Radical - antagonists & inhibitors; Hydroxyl Radical - metabolism; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Liquid-Liquid Extraction - methods; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; Medicine; Medicine & Public Health; Mice; Mice, Inbred Strains; Oxidation-Reduction; Oxidative Stress - drug effects; Phaeophyceae - chemistry; Sargassum - chemistry; Sargassum hemiphyllum; Superoxides - antagonists & inhibitors; Superoxides - metabolism; Tannins - isolation & purification; Tannins - pharmacology; Vitamin E - pharmacology; 多酚; 抗氧化成分; 抗氧化活性; 抗氧化能力; 清除能力; 生物活性化合物; 羟基自由基; antioxidant activities; phlorotannins; in vivo; Sargassum hemiphyllum; in vitro; phlorotarmins
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-016-1607-6

Brown algae are well known as a source of biologically active compounds, especially those having antioxidant activities, such as phlorotannins. In this study we examined the antioxidant activities of crude phlorotannins extracts（CPEs） obtained from Sargassum hemiphyllum（SH） and fractionated according to the molecular weights. When CPEs were administrated at a dose of 30 mg/kg to Kunming mice pre-treated with carbon tetrachloride（CCl4）, the levels of oxidative stress indicators in the liver, kidney and brain were significantly reduced in vivo. All the components of various molecular weight fractions of CPEs exhibited greater scavenging capacities in clearing hydroxyl free radical and superoxide anion than the positive controls gallic acid, vitamin C and vitamin E. Particularly, the components greater than 30 k D obtained from ethyl acetate phase showed the highest antioxidant capacities. These results indicated that SH is a potential source for extracting phlorotannins, the algal antioxidant compounds.