Fecal Microbiota Transplantation from Mice Receiving Magnetic Mitohormesis Treatment Reverses High-Fat Diet-Induced Metabolic and Osteogenic Dysfunction

• Published in: International journal of molecular sciences Vol. 26; no. 12; p. 5450
• Main Authors: Wong, Jun Kit Craig, Patel, Bharati Kadamb, Tai, Yee Kit, Tan, Tuan Zea, Khine, Wei Wei Thwe, Chen, Way Cherng, Kukumberg, Marek, Ching, Jianhong, Lee, Lye Siang, Chua, Kee Voon, Tan, Tsze Yin, Wu, Kwan Yu, Bai, Xizhe, Iversen, Jan Nikolas, Purnamawati, Kristy, Abdul Jalil, Rufaihah, Kumar, Alan Prem, Lee, Yuan Kun, Moochhala, Shabbir M., Franco-Obregón, Alfredo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.06.2025; MDPI
• Subjects: Animals; Antibiotics; Body fat; Bone Density; Bones; Ceramides; Ceramides - metabolism; Comparative analysis; Density; Dextrose; Diet; Diet, High-Fat - adverse effects; Electromagnetic fields; Electromagnetism; Fatty acids; Fecal Microbiota Transplantation - methods; Feces; Gastrointestinal Microbiome; Gene expression; Genes; Genotype & phenotype; Glucose; Inflammation; Intervention; Liver - metabolism; Magnetic Field Therapy - methods; Male; Metabolic Diseases - etiology; Metabolic Diseases - metabolism; Metabolic Diseases - therapy; Metabolism; Mice; Mice, Inbred C57BL; Microbiota; Microbiota (Symbiotic organisms); Obesity; Osteogenesis; Osteoporosis; Plant lipids; Type 2 diabetes; Singapore; California; United States; Massachusetts; non-alcoholic fatty liver disease (NAFLD); gut microbiome; non-alcoholic steatohepatitis (NASH); osteogenesis; PEMF therapy; ceramides; obesity
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms26125450

This study compared the metabolic consequences of fecal microbiota transplantation (FMT) from donor mice that had been either administered pulsed electromagnetic field (PEMF) therapy or exercised to recipient mice fed a high-fat diet (HFD). Eight weeks of PEMF treatment (10 min/week) enhanced PGC-1α-associated mitochondrial and metabolic gene expression in white and brown adipose to a greater degree than eight weeks of exercise (30–40 min/week). FMT from PEMF-treated donor mice recapitulated these adipogenic adaptations in HFD-fed recipient mice more faithfully than FMT from exercised donors. Direct PEMF treatment altered hepatic phospholipid composition, reducing long-chain ceramides (C16:0) and increasing very long-chain ceramides (C24:0), which could be transferred to PEMF-FMT recipient mice. FMT from PEMF-treated mice was also more effective at recovering glucose tolerance than FMT from exercised mice. PEMF treatment also enhanced bone density in both donor and HFD recipient mice. The gut Firmicutes/Bacteroidetes (F/B) ratio was lowest in both the directly PEMF-exposed and PEMF-FMT recipient mouse groups, consistent with a leaner phenotype. PEMF treatment, either directly applied or via FMT, enhanced adipose thermogenesis, ceramide levels, bone density, hepatic lipids, F/B ratio, and inflammatory blood biomarkers more than exercise. PEMF therapy may represent a non-invasive and non-strenuous method to ameliorate metabolic disorders.