Anti-inflammatory and anti-oxidative effect of Korean propolis on Helicobacter pylori-induced gastric damage in vitro

• Published in: The journal of microbiology Vol. 58; no. 10; pp. 878 - 885
• Main Authors: Song, Moon-Young, Lee, Da-Young, Kim, Eun-Hee
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.10.2020; Springer Nature B.V; 한국미생물학회
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; Adenocarcinoma; Anti-Inflammatory Agents - pharmacology; antioxidant activity; Antioxidants; Antioxidants - pharmacology; Apiculture; bacteria; Beekeeping; Biomedical and Life Sciences; Cell Line, Tumor; Cyclooxygenase-2; Cytokines - metabolism; Damage; dose response; Free radicals; Gastritis; Gastritis - drug therapy; Gastritis - microbiology; Gastritis - pathology; Gastrointestinal diseases; Helicobacter Infections - pathology; Helicobacter pylori; Helicobacter pylori - pathogenicity; Humans; Hydrates; IL-1β; inducible nitric oxide synthase; Inflammation; Interleukin 12; Interleukin 8; Kinases; Life Sciences; Microbiology; mitogen-activated protein kinase; Necrosis; Neoplasms; NF-E2-Related Factor 2 - metabolism; NF-kappa B - metabolism; NF-κB protein; Nitric oxide; Nitric-oxide synthase; Oxidants; Oxidative Stress - drug effects; Oxidizing agents; Peptic Ulcer - drug therapy; Peptic Ulcer - microbiology; Peptic Ulcer - pathology; Peptic ulcers; Pretreatment; Propolis; Propolis - pharmacology; prostaglandin synthase; Reactive oxygen species; Republic of Korea; Resins; Scavenging; Signal Transduction - drug effects; South Korea; Stomach; tumor necrosis factor-alpha; Tumor necrosis factor-α; Ulcers; 생물학; Republic of Korea; South Korea; NF-κB; anti-oxidant; anti-inflammation; Nrf2; Korean propolis; Helicobacter pylori
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-020-0277-z

Helicobacter pylori , present in the stomach lining, is a Gramnegative bacterium that causes various gastrointestinal diseases, including gastritis and peptic ulcers. Propolis is a natural resinous substance collected from a variety of plants, and contains several natural bioactive substances. The aim of this study was to investigate the anti-inflammatory and antioxidative effects of Korean propolis on H. pylori -induced damage in the human adenocarcinoma gastric cell line. The propolis used in this study was obtained from the Korea Beekeeping Association in South Korea. The expression of pro-inflammatory interleukins (ILs), such as IL-8, IL-12, IL-1β, tumor necrosis factor alpha, cyclooxygenase-2, and inducible nitric oxide synthase, which was increased after H. pylori infection, significantly decreased in a dose-dependent manner upon pretreatment with Korean propolis, because of the suppression of mitogen-activated protein kinases and nuclear factor kB pathway. The anti-oxidative activity of propolis was assessed using the 2,2-diphenyl-1-picrylhydrazyl hydrate free radical assay. Korean propolis showed significant anti-oxidative effects via reactive oxygen species scavenging. In addition, pretreatment with Korean propolis upregulated the expression of anti-oxidant enzymes through Nrf2 signaling activation. These findings indicate that the use of Korean propolis, which has anti-inflammatory and anti-oxidative effects, can be promising for the prevention of H. pylori -induced gastric damage.