Assessment of the Number and Function of Macrophages in the Placenta of Gestational Diabetes Mellitus Patients

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 33; no. 5; pp. 725 - 729
• Main Author: 余俊 周勇 桂娟 李艾珍 宿晓玲 冯玲
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2013; Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China
• Subjects: Adult; Antigens, CD; Antigens, Differentiation, Myelomonocytic; Cell Count; Cytokines - genetics; Cytokines - immunology; Cytokines - metabolism; Diabetes, Gestational - genetics; Diabetes, Gestational - immunology; Diabetes, Gestational - metabolism; Female; Humans; Immunohistochemistry; Inflammation Mediators - immunology; Inflammation Mediators - metabolism; Interleukin-6 - genetics; Interleukin-6 - immunology; Interleukin-6 - metabolism; Lipopolysaccharide Receptors - genetics; Lipopolysaccharide Receptors - metabolism; Macrophages - immunology; Macrophages - metabolism; Macrophages - pathology; Medicine; Medicine & Public Health; Placenta - immunology; Placenta - metabolism; Pregnancy; Reverse Transcriptase Polymerase Chain Reaction; TNF-α; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Tumor Necrosis Factor-alpha - metabolism; 功能评估; 妊娠期; 巨噬细胞; 患者; 炎性细胞因子; 糖尿病; 胎盘组织; macrophage; placenta; gestational diabetes mellitus; pro-inflammation factors
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-013-1187-7

In order to assess the number and function of macrophages in the placenta of pregnancy complicated with gestational diabetes mellitus （GDM） as well as those of normal pregnancies, placenta samples were collected from 15 GDM patients （GDM group） and 10 normal pregnant women （control group）. The expression levels of macrophage markers （CD68/CD14） and inflammatory cytokines （IL-6/TNF-α） in placenta were detected using immunohistochemistry and PCR. The results showed that the number of CD68＋ or CD14＋ cells in the GMD group was remarkably higher than that in the control group （P〈0.05）, indicating that the number of macrophages in the GDM group was significantly greater than that in the control group. The mRNA expression levels of CD68＋, IL-6 and TNF-α were higher in the GMD group than in the control group. In conclusion, more macrophages accumulate in placenta of pregnancy complicated with GDM, and the expression levels of pro-inflammation factors are also in- creased in GDM pregnancies, suggesting that macrophages and inflammatory mediators （IL-6 and TNF-α） mav olav an imoortant role in GDM.