Genes Related to Intracellular Survival of Brucella abortus in THP-1 Macrophage Cells

• Published in: Journal of microbiology and biotechnology Vol. 28; no. 10; pp. 1736 - 1748
• Main Authors: Shim, Soojin, Im, Young Bin, Jung, Myunghwan, Park, Woo Bin, Yoo, Han Sang
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.10.2018
• Subjects: Brucella abortus - genetics; Brucella abortus - growth & development; Brucella abortus - physiology; Cytokines - analysis; Cytoplasm - microbiology; Gene Deletion; Gene Expression Regulation; Gene Regulatory Networks - genetics; Genes, Bacterial - genetics; Host Microbial Interactions; Humans; Macrophages - microbiology; Microarray Analysis; Microbial Viability; Mutagenesis, Insertional; THP-1 Cells; 생물학; THP-1 macrophage cells; intracellular survival and replication; B. abortus; mutants; gene expression
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1805.05068

can survive and replicate within host macrophages, and great efforts have been made to demonstrate the genes involved in pathogenicity, such as internalization, in research. Here, intracellular responses were compared between THP-1 macrophage cells stimulated with wild-type and four mutants (C1, C10, C27, and C32) using microarray to demonstrate the role of genes related to intracellular survival and replication. These mutants were generated by deleting genes encoding (4-hydrobenzoate 3-monooxygenase, PHBH), (heme exporter protein cytochrome C, CcmC), (exopolyphosphatase, PPX), and (peptidase M24). The results showed that mutants C1 and C10 induced significant suppression of survival levels and cytokine expression relative to wild-type in the THP-1 macrophage cells. These findings suggest that the and genes play important roles in survival within human macrophages. Conversely, mutants C27 and C32 induced significantly higher survival level than wild-type in the cells inhibiting cellular signal transduction. It is assumed that the and genes play a role in cellular resistance to . Therefore, the disrupted genes are involved in intracellular growth, and especially in its survival, and they could be effective targets for understanding the intracellular bacterium, .