Recent Developments in Correlative Super-Resolution Fluorescence Microscopy and Electron Microscopy

• Published in: Molecules and cells Vol. 45; no. 1; pp. 41 - 50
• Main Authors: Jeong, Dokyung, Kim, Doory
• Format: Journal Article
• Language: English
• Published: United States Korean Society for Molecular and Cellular Biology 31.01.2022; 한국분자세포생물학회
• Subjects: Microscopy, Electron; Microscopy, Fluorescence - methods; Minireview; 생물학; electron microscopy; correlative super-resolution fluorescence and electron microscopy; correlative light and electron microscopy; super-resolution fluorescence microscopy
• ISSN: 1016-8478; 0219-1032
• DOI: 10.14348/molcells.2021.5011

The recently developed correlative super-resolution fluorescence microscopy (SRM) and electron microscopy (EM) is a hybrid technique that simultaneously obtains the spatial locations of specific molecules with SRM and the context of the cellular ultrastructure by EM. Although the combination of SRM and EM remains challenging owing to the incompatibility of samples prepared for these techniques, the increasing research attention on these methods has led to drastic improvements in their performances and resulted in wide applications. Here, we review the development of correlative SRM and EM (sCLEM) with a focus on the correlation of EM with different SRM techniques. We discuss the limitations of the integration of these two microscopy techniques and how these challenges can be addressed to improve the quality of correlative images. Finally, we address possible future improvements and advances in the continued development and wide application of sCLEM approaches.