Characterization of a Salmonella Enteritidis bacteriophage showing broad lytic activity against Gram-negative enteric bacteria

• Published in: The journal of microbiology Vol. 56; no. 12; pp. 917 - 925
• Main Authors: Kim, Shukho, Kim, Sung-Hun, Rahman, Marzia, Kim, Jungmin
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.12.2018; Springer Nature B.V; 한국미생물학회
• Subjects: Animals; Bacteria; Bacterial diseases; bacterial infections; Bacteriophages; Base pairs; Biomedical and Life Sciences; Democratic People's Republic of Korea; E coli; Enterobacter cloacae; Enterobacter cloacae - virology; Enterobacteriaceae - virology; Escherichia coli; Escherichia coli - virology; Farms; food pathogens; Foodborne Diseases - microbiology; Foodborne pathogens; Gastrointestinal Microbiome; genome; Genome, Viral; Genomes; Gram-negative bacteria; Host Specificity; Icosahedral phase; intestinal microorganisms; Life Sciences; Lysozyme; Microbiology; Open reading frames; Open Reading Frames - genetics; Pathogenic bacteria; Pathogens; Phages; Phylogeny; Poultry; Proteins; Salmonella; Salmonella enterica; Salmonella enterica - virology; Salmonella Enteritidis; Salmonella enteritidis - virology; Salmonella Phages - classification; Salmonella Phages - genetics; Salmonella Phages - isolation & purification; Salmonella Phages - physiology; Sequence Analysis, DNA; sequence homology; serotypes; Serratia marcescens; Sewage - virology; Shigella sonnei; Shigella sonnei - drug effects; Siphoviridae; Specificity; Spike protein; viral morphology; virulent strains; 생물학; Democratic People's Republic of Korea; foodborne pathogens; bacteriophage; Salmonella
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-018-8310-1

In this study, we sought to isolate Salmonella Enteritidis-specific lytic bacteriophages (phages), and we found a lytic phage that could lyse not only S. Enteritidis but also other Gramnegative foodborne pathogens. This lytic phage, SS3e, could lyse almost all tested Salmonella enterica serovars as well as other enteric pathogenic bacteria including Escherichia coli , Shigella sonnei , Enterobacter cloacae , and Serratia marcescens . This SS3e phage has an icosahedral head and a long tail, indicating belong to the Siphoviridae . The genome was 40,793 base pairs, containing 58 theoretically determined open reading frames (ORFs). Among the 58 ORFs, ORF49, and ORF25 showed high sequence similarity with tail spike protein and lysozyme-like protein of Salmonella phage SE2, respectively, which are critical proteins recognizing and lysing host bacteria. Unlike SE2 phage whose host restricted to Salmonella enterica serovars Enteritidis and Gallinarum, SS3e showed broader host specificity against Gram-negative enteric bacteria; thus, it could be a promising candidate for the phage utilization against various Gram-negative bacterial infection including foodborne pathogens.