Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters

• Published in: The Journal of physiology Vol. 600; no. 10; pp. 2377 - 2400
• Main Authors: Vacca, Francesca, Gomes, Ana S., Murashita, Koji, Cinquetti, Raffella, Roseti, Cristina, Barca, Amilcare, Rønnestad, Ivar, Verri, Tiziano, Bossi, Elena
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.05.2022; John Wiley and Sons Inc
• Subjects: Affinity; Danio rerio; Di/tripeptide transport(ers); Gastrointestinal tract; Gene duplication; Genomes; Gills; Kidneys; Molecular and Cellular; Neural coding; Oocytes; PepT2a; PepT2b; presteady‐state currents; Proteins; Salmo salar; Salmon; Slc15a2a; Slc15a2b; two‐electrode voltage‐clamp; whole‐genome duplication; Xenopus laevis oocytes; Xenopus laevis oocytes; presteady-state currents; whole-genome duplication; Di/tripeptide transport(ers); Slc15a2a; Slc15a2b; two-electrode voltage-clamp; PepT2b; PepT2a
• ISSN: 0022-3751; 1469-7793; 1469-7793
• DOI: 10.1113/JP282781

The high‐affinity/low‐capacity system Slc15a2 (PepT2) is responsible for the reuptake of di/tripeptides from the renal proximal tubule, but it also operates in many other tissues and organs. Information regarding PepT2 in teleost fish is limited and, to date, functional data are available from the zebrafish (Danio rerio) only. Here, we report the identification of two slc15a2 genes in the Atlantic salmon (Salmo salar) genome, namely slc15a2a and slc15a2b. The two encoded PepT2 proteins share 87% identity and resemble both structurally and functionally the canonical vertebrate PepT2 system. The mRNA tissue distribution analyses reveal a widespread distribution of slc15a2a transcripts, being more abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and the distal part of the gastrointestinal tract. The function of the two transporters was investigated by heterologous expression in Xenopus laevis oocytes and two‐electrode voltage‐clamp recordings of transport and presteady‐state currents. Both PepT2a and PepT2b in the presence of Gly‐Gln elicit pH‐dependent and Na+ independent inward currents. The biophysical and kinetic analysis of the recorded currents defined the transport properties, confirming that the two Atlantic salmon PepT2 proteins behave as high‐affinity/low‐capacity transporters. The recent structures and the previous kinetic schemes of rat and human PepT2 qualitatively account for the characteristics of the two Atlantic salmon proteins. This study is the first to report on the functional expression of two PepT2‐type transporters that operate in the same vertebrate organism as a result of (a) gene duplication process(es). Key points Two slc15a2‐type genes, slc15a2a and slc15a2b coding for PepT2‐type peptide transporters were found in the Atlantic salmon. slc15a2a transcripts, widely distributed in the fish tissues, are abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and distal gastrointestinal tract. Amino acids involved in vertebrate Slc15 transport function are conserved in PepT2a and PepT2b proteins. Detailed kinetic analysis indicates that both PepT2a and PepT2b operate as high‐affinity transporters. The kinetic schemes and structures proposed for the mammalian models of PepT2 are suitable to explain the function of the two Atlantic salmon transporters. figure legend Two genes coding for high‐affinity/low‐capacity system Slc15a2 (PepT2), are present in the Atlantic salmon (Salmo salar) genome: slc15a2a and slc15a2b. The two encoded PepT2 proteins share 87% identity and resemble both structurally and functionally the canonical vertebrate PepT2 system. Heterologous expression in Xenopus laevis oocytes and two‐electrode voltage‐clamp were applied to record transport and presteady‐state currents to characterize the function of the two transporters. The transport properties collected confirmed that the previous kinetic schemes of rat and human PepT2 qualitatively account for the characteristics of the two Atlantic salmon proteins. This study is the first to report on the functional expression of two PepT2‐type transporters that operate in the same vertebrate organism as a result of (a) gene duplication process(es).