Heme Oxygenase-1 Induction and Anti-inflammatory Actions of Atractylodes macrocephala and Taraxacum herba Extracts Prevented Colitis and Was More Effective than Sulfasalazine in Preventing Relapse

• Published in: Gut and liver Vol. 11; no. 5; pp. 655 - 666
• Main Authors: Han, Kyu-Hyun, Park, Jong-Min, Jeong, Migyeong, Han, Young-Min, Go, Eun-Jin, Park, Juyeon, Kim, Hocheol, Han, Jae Gab, Kwon, Oran, Hahm, Ki Baik
• Format: Journal Article
• Language: English
• Published: Korea (South) Editorial Office of Gut and Liver 01.09.2017; Gastroenterology Council for Gut and Liver; 거트앤리버 소화기연관학회협의회
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Atractylodes - chemistry; atractylodes macrocephala; Colitis - chemically induced; Colitis - drug therapy; Colitis - prevention & control; Cyclooxygenase 2 - biosynthesis; Dextran Sulfate; Enzyme Induction - drug effects; Heme Oxygenase-1 - biosynthesis; inflammation; Inflammation Mediators - antagonists & inhibitors; inflammatory bowel disease; Lipopolysaccharides; Male; Membrane Proteins - biosynthesis; Mice; Mice, Inbred C57BL; Original; Phytotherapy; Plant Extracts - pharmacology; regeneration; Secondary Prevention - methods; Sulfasalazine - pharmacology; Taraxacum - chemistry; taraxacum herba; Tumor Necrosis Factor-alpha - biosynthesis; 내과학; Inflammatory bowel disease; Regeneration; Inflammation; Atractylodes macrocephala; Taraxacum herba
• ISSN: 1976-2283; 2005-1212; 2005-1212
• DOI: 10.5009/gnl16496

In inflammatory bowel disease (IBD), repeated bouts of remission and relapse occur in patients and can impose a risk of colitis-associated cancer. We hypothesized that plant extracts of (AM) or (TH) may be better than sulfasalazine for treating this disease because these extracts can promote additional regeneration. Murine intestinal epithelial IEC-6 cells were pretreated with AM or TH before a lipopolysaccharide (LPS)-induced challenge. Acute colitis was induced with 7 days of dextran sulfate sodium (DSS) in male C57BL/6 mice, and extracts of AM and TH were administered for 2 weeks before DSS administration. studies demonstrated that AM or TH treatment reduced LPS-induced -2 and -α mRNA levels but increased heme oxygenase-1 (HO-1). Oral preadministration of AM and TH rescued mice from DSS-induced colitis by inhibiting inflammatory mediators via inactivated extracellular signal regulated kinase and repressed nuclear factor κB and signal transducer and activator of transcription 3, but the effect was weaker for sulfasalazine than that for the extracts. Anti-inflammatory activities occurred via the inhibition of macrophage and T lymphocyte infiltrations. Unlike sulfasalazine, which did not induce HO-1, TH extracts afforded significant HO-1 induction. Because the AM or TH extracts were far superior in preventing DSS-induced colitis than sulfasalazine, AM or TH extracts can be considered natural agents that can prevent IBD relapse.