Effects of Spermidine and p-Cresol on Polymorphonuclear Cell Apoptosis and Function

• Published in: Artificial organs Vol. 35; no. 2; pp. E27 - E32
• Main Authors: de Carvalho Jr, Jose Tarcisio G., Dalboni, Maria A., Watanabe, Renato, Peres, Alines T., Goes, Miguel A., Manfredi, Silvia R., Canziani, Maria E., Cendoroglo, Gabriel S., Guimarães-Souza, Nadia, Batista, Marcelo C., Cendoroglo, Miguel
• Format: Journal Article
• Language: English
• Published: Malden, USA Blackwell Publishing Inc 01.02.2011
• Subjects: Apoptosis; Apoptosis - drug effects; CD11b Antigen - immunology; Cresols - pharmacology; Humans; Immune dysfunction; Neutrophils - cytology; Neutrophils - drug effects; Neutrophils - immunology; Neutrophils - metabolism; Reactive Oxygen Species - metabolism; Reactive oxygen species production; Spermidine - pharmacology; Uremic toxins
• ISSN: 0160-564X; 1525-1594; 1525-1594
• DOI: 10.1111/j.1525-1594.2010.01116.x

Polymorphonuclear leukocytes (PMNs) from chronic kidney disease (CKD) patients display accelerated apoptosis and dysfunction, which may predispose CKD patients to infections. In this study, we investigated the effect of spermidine and p‐cresol on apoptosis and function on PMN from healthy subjects. We measured the effect of spermidine and p‐cresol on apoptosis, ROS production unstimulated and stimulated (S. aureus and PMA) and expression of CD95, caspase 3, and CD11b on PMN. After incubation with p‐cresol and spermidine, we did not observe any changes in apoptosis, viability or expression of caspase 3 and CD95 in PMN from healthy subjects. PMN incubated for 10 minutes with spermidine demonstrated a significant reduction in spontaneous, S. aureus and PMA‐stimulated ROS production. p‐cresol induced a decrease in PMA‐stimulated ROS production. Spermidine and p‐cresol also induced a decrease in the expression of CD11b on PMN. Spermidine and p‐cresol decreased the expression of CD11b and oxidative burst of PMN from healthy subjects and had no effect on PMN apoptosis and viability.