Local Expression of Vaginal Th1 and Th2 Cytokines in Murine Vaginal Candidiasis under Different Immunity Conditions

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 28; no. 4; pp. 476 - 479
• Main Author: 陈善娟 李少华 吴艳 刘志香 李家文
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.08.2008; Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China%Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Animals; Candida albicans - isolation & purification; Candidia; Candidiasis, Vulvovaginal - immunology; Candidiasis, Vulvovaginal - microbiology; Cytokines - genetics; Cytokines - metabolism; Estrogens - pharmacology; Female; Immunocompromised Host - immunology; Interleukin-10 - genetics; Interleukin-10 - metabolism; Interleukin-2 - genetics; Interleukin-2 - metabolism; Interleukin-4 - genetics; Interleukin-4 - metabolism; Medicine; Medicine & Public Health; Mice; Mice, Inbred ICR; RNA, Messenger - genetics; RNA, Messenger - metabolism; Th1 Cells - immunology; Th1/Th2; Th2 Cells - immunology; Vagina - immunology; 白念珠菌; 阴道冲洗; vaginal lavage; murine model; immuno-suppression; Th1/Th2 cytokinese; Candida albicans
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-008-0423-z

To investigate the expression of vaginal Th1 and Th2 cytokines in rats with experimental vaginal candidiasis under different immune conditions, ICR murine vaginal candidiasis model was established and immno-suppressed murine models of vaginal cadidiasis were established in estrogen-treated mice. Non-estrogen-treated mice were used as controls. The mRNA level of Th1 （IL-2）/Th2 （IL-4, IL-10, TGF-β1） cytokines in murine vaginal tissues was determined by RT-PCR. The cykotine in local tissues was increased to different extent under normal immune condition. IL-2 mRNA was increased during early stage of infection, while IL-10 was increased transiently during late stage of infection. TGF-β1 production was found to be increased persistently. At same time, the expression of IL-2 mRNA was suppressed in immno-suppressed group, and the level of IL-4, IL-10, and TGF-β1 were higher than the normal immunity group to different degree during infection. The high level of IL-2 mRNA during early stage of infection was associated with clearance of mucosal Candidia albicans （C. albicans）, and its expression suppressed leading to decreased clearance of mucosal C. albican in immuno-suppression. The over-expression of IL-4 and IL-10 could significantly enhance the susceptibility to C. albicans infection in mice.