Effects of Panaxadiol Saponins Component as A New Chinese Patent Medicine on Proliferation, Differentiation and Corresponding Gene Expression Profile of Megakaryocytes

• Published in: Chinese journal of integrative medicine Vol. 22; no. 1; pp. 28 - 35
• Main Author: 温伟为 孙馨 庄海峰 林筱洁 郑智茵 高瑞兰 尹利明
• Format: Journal Article
• Language: English
• Published: Beijing Chinese Association of Traditional and Western Medicine 01.01.2016
• Subjects: Blotting, Western; Bone Marrow Cells - cytology; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Colony-Forming Units Assay; Drugs, Chinese Herbal - pharmacology; Flow Cytometry; Gene Expression Profiling; Ginsenosides - pharmacology; Humans; Medicine; Medicine & Public Health; Megakaryocytes - cytology; Megakaryocytes - drug effects; Megakaryocytes - metabolism; Original Article; Patents as Topic; Saponins - pharmacology; Stem Cells - cytology; Stem Cells - drug effects; Transcription Factors - metabolism; Up-Regulation - drug effects; Up-Regulation - genetics; 人参二醇; 人参总皂苷; 体外分化; 基因表达谱; 巨核细胞; 有效成分; 流式细胞仪分析; 细胞增殖; megakaryocyte; panaxadiol saponins; gene expression profile; differentiation; proliferation
• ISSN: 1672-0415; 1993-0402
• DOI: 10.1007/s11655-015-1970-3

Objective： To investigate the effects of panaxadiol saponins component （PDS-C） isolated from total saponins of panax ginseng on proliferation, differentiation and corresponding gone expression profile of megakaryocytes. Methods： Bone marrow culture of colony forming assay of megakaryocytic progenitor cells （CFU-MK） was observed for the promoting proliferation mediated by PDS-C, and differentiation of megakaryocytic blasts caused by PDS-C was analyzed with flow cytometry in CHRF-288 and Meg-01 cells, as well as proliferation, differentiation-related genes expression profile and protein expression levels were detected by human gone expression microarray and western blot. Results： In response to PDS-C 10, 20 and 50 mg/L, CFU-MK from 10 human bone marrow samples was increased by 28.9± 2.7%, 41.0% ± 3.2% and 40.5% ± 2.6% over untreated control, respectively （P〈0.01, each）. Flow cytometry analysis showed that PDS-C treated CHRF-288 cells and Meg-01 cells significantly increased in CD42b, CD41, TSP and CD36 positive ratio, respectively. PDS-C induced 29 genes up-regulated more than two-fold commonly in both cells detected by human expression microarray representing 4000 known genes. The protein expression levels of ZNF91, c-Fos, BTF3a, GATA-1, RGS2, NDRG2 and RUNX1 were increased with western blot in correspond to microarray results. Conclusion： PDS-C as an effective component for hematopoiesis, play the role to enhance proliferation and differentiation of megakaryocytes, also up-regulated expression of proliferation, differentiation-related genes and proteins in vitro. KEYWORDS panaxadiol saponins, megakaryocyte, gone expression profile, proliferation, differentiation