Isolation of a Family of Organic Anion Transporters from Human Liver and Kidney

• Published in: Biochemical and biophysical research communications Vol. 283; no. 2; pp. 417 - 422
• Main Authors: Sun, William, Wu, Rong Rong, van Poelje, Paul D., Erion, Mark D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.05.2001
• Subjects: a-ketoglutarate; Amino Acid Sequence; Animals; Anion Transport Proteins; Carrier Proteins - genetics; Carrier Proteins - isolation & purification; Carrier Proteins - metabolism; Cell Line; Cloning, Molecular; Cyclic AMP - metabolism; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Female; fluorescein; hOAT1 gene; hOAT2 gene; hOAT3 gene; hOAT4 gene; hOAT5 gene; Humans; In Vitro Techniques; Ketoglutaric Acids - metabolism; Kidney - metabolism; kidney transport; Liver - metabolism; liver transport; membrane transport; methotrexate; Methotrexate - metabolism; Molecular Sequence Data; Oocytes - metabolism; organic anion; Organic Anion Transporters; Organic Anion Transporters, Sodium-Independent; p-aminohippurate; p-Aminohippuric Acid - metabolism; Rats; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Species Specificity; Transfection; Xenopus laevis; kidney transport; membrane transport; organic anion; liver transport; p-aminohippurate
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2001.4774

Five distinct organic anion transporter cDNAs, hOAT1-5, were isolated from human liver and kidney. hOAT1, 2, and 3 are homologous to their respective rat orthologues OAT1-3, whereas hOAT4 and 5 are novel clones that have not been identified in other species. hOAT1- and hOAT3-transfected cells showed uptake of p-aminohippurate and fluorescein. Cells expressing hOAT2 showed uptake of p-aminohippurate, methotrexate, cAMP, and α-ketoglutarate. Northern blot analysis indicated differential tissue distribution for the transporter transcripts. These results indicate the existence of a family of organic anion transporting proteins in humans distinct from the oatp-like family of transporters.