Proliferative and apoptotic effects of andrographolide on the BGC-823 human gastric cancer cell line

• Published in: Chinese medical journal Vol. 126; no. 19; pp. 3739 - 3744
• Main Authors: Li, Shu-guang, Wang, Yuan-yu, Ye, Zai-yuan, Shao, Qing-shu, Tao, Hou-quan, Shu, Li-sha, Zhao, Yi-feng, Yang, Yong-jiang, Yang, Jing, Peng, Tao, Han, Bo, Huang, Di
• Format: Journal Article
• Language: English
• Published: China Department of Gastrointestinal Surgery, the First Affiliated Hospital, Hebei North University, Zhangjiakou, Hebei 075000, China%Department of Gastrointestinal Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, China 2013
• Subjects: Apoptosis - drug effects; Bcl-2; bcl-2-Associated X Protein - analysis; bcl-2-Associated X Protein - genetics; BGC-823; Caspase 3 - analysis; Caspase 3 - genetics; caspase-3; Cell Cycle - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Diterpenes - pharmacology; Dose-Response Relationship, Drug; Humans; Proto-Oncogene Proteins c-bcl-2 - analysis; Stomach Neoplasms - drug therapy; Stomach Neoplasms - pathology; 穿心莲内酯; 细胞凋亡; 细胞增殖; 细胞株; 胃癌; apoptosis; tumor cells; cell cycle; proliferative inhibition; andrographolide
• ISSN: 0366-6999; 2542-5641; 2542-5641
• DOI: 10.3760/cma.j.issn.0366-6999.20130458

Background Andrographolide has been shown to have anticancer activity on diverse cancer cell lines representing different types of human cancers.The aim of this research was to investigate the anticancer and apoptotic effects of andrographolide on the BGC-823 human gastric cancer cell line.Methods Cell proliferation and IC50 were evaluated using MTT assay,cell-cycle analysis with flow cytometry apoptotic effects with Annexin-V/propidium iodide double-staining assay,and morphologic structure with transmission electron microscopy.Immunohistochemistry and reverse-transcription PCR was used to analyze Bcl-2,Bax,and caspase-3 expressions.Results Andrographolide showed a time-and concentration-dependent inhibitory effects on BGC-823 cell growth.Compared to controls,the number of cells in the G0-G1-phase increased significantly,S and G2-M-phase cells decreased after 48 hours of treatment with andrographolide,and both early and late apoptotic rates increased significantly compared to the controls,all in a concentration-dependent manner.Bax and caspase-3 expressions were markedly increased,and Bcl-2 expression was decreased.Conclusions Andrographolide inhibits BGC-823 cell growth and induces BGC-823 cell apoptosis by up-regulating Bax and caspase-3 expressions and down-regulating Bcl-2 expression.Andrographolide may be useful as a potent and selective agent in the treatment of human gastric cancers.