Upregulation of miR-23b Enhances the Autologous Therapeutic Potential for Degenerative Arthritis by Targeting PRKACB in Synovial Fluid-Derived Mesenchymal Stem Cells from Patients

• Published in: Molecules and cells Vol. 37; no. 6; pp. 449 - 456
• Main Authors: Ham, Onju, Lee, Chang Youn, Song, Byeong-Wook, Lee, Se-Yeon, Kim, Ran, Park, Jun-Hee, Lee, Jiyun, Seo, Hyang-Hee, Lee, Chae Yoon, Chung, Yong-An, Maeng, Lee-So, Lee, Min Young, Kim, Jongmin, Hwang, Jihwan, Woo, Dong Kyun, Chang, Woochul
• Format: Journal Article
• Language: English
• Published: United States Korean Society for Molecular and Cellular Biology 01.06.2014; 한국분자세포생물학회
• Subjects: Cell Differentiation - drug effects; Cells, Cultured; Chondrocytes - metabolism; Collagenases - metabolism; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits - antagonists & inhibitors; Gene Expression Regulation; HeLa Cells; Humans; Isoquinolines - pharmacology; Mesenchymal Stem Cell Transplantation - methods; MicroRNAs - genetics; Osteoarthritis - genetics; Osteoarthritis - therapy; Sulfonamides - pharmacology; Synovial Fluid - cytology; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.14348/molcells.2014.0023

The use of synovial fluid-derived mesenchymal stem cells (SFMSCs) obtained from patients with degenerative arthropathy may serve as an alternative therapeutic strategy in osteoarthritis (OA) and rheumatoid arthritis (RA). For treatment of OA and RA patients, autologous transplantation of differentiated MSCs has several beneficial effects for cartilage regeneration including immunomodulatory activity. In this study, we induced chondrogenic differentiation of SFMSCs by inhibiting protein kinase A (PKA) with a small molecule and microRNA (miRNA). Chondrogenic differentiation was confirmed by PCR and immunocytochemistry using probes specific for aggrecan, the major cartilaginous proteoglycan gene. Absorbance of alcian blue stain to detect chondrogenic differentiation was increased in H-89 and/or miRNA-23btransfected cells. Furthermore, expression of matrix metalloproteinase (MMP)-9 and MMP-2 was decreased in treated cells. Therefore, differentiation of SFMSCs into chondrocytes through inhibition of PKA signaling may be a therapeutic option for OA or RA patients.