Expression of Transcription Factor Oct4 in Bladder Cancer Cell Line T24 and Its Effects on the Biological Characteristics of the Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 29; no. 1; pp. 73 - 76
• Main Author: 朱志强 文建国 郑湘予 王道协 王庆伟 樊长辉
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2009; Department of Emergency Center and Pathology,The First Hospital of Zhengzhou University,Institute of Clinical Medical Research of Henan Universities,Zhengzhou 450052,China
• Subjects: Boyden小室; Cell Line, Tumor; Cell Proliferation; Humans; Medicine; Medicine & Public Health; Oct4; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; PCR技术; Reverse Transcriptase Polymerase Chain Reaction; RNA, Small Interfering - genetics; Transwell; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; 基因沉默; 生物学特性; 膀胱癌T24细胞; 转录因子; siRNA; Octamer binding factor 4; bladder cancer; T24 cells
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-009-0115-3

The expression of octamer binding factor 4 （Oct4） gene in bladder cancer cell line T24 and its effects on the biological characteristics of the cells were investigated. RT-PCR and Western blot were employed to detect the expression of Oct4 in T24 cells. The changes of biological characteristics in T24 cells were analyzed before and after gene-silencing by Boyden chamber and MTT. The results showed that the expression of Oct4 gene was detectable in T24 cells by RT-PCR and Western blot. The expression of Oct4 gene and protein was down-regulated by siRNA, and average number of transwell cells in interference group, negative control group and blank control group was 101.40±54.56, 104.20± 10.03 and 111.00±11.90, respectively. There was significant difference in the proliferation abilit,） of the cells from 48 h, 72 h to 96 h after the interference by siRNA between interference group and negative group or blank control group （P〈0.05）. It was suggested that Oct4 gene was related with proliferation ability of T24 cells, but not with invasive capability.