Comparative study of total lipids in beef using chlorinated solvent and low-toxicity solvent methods

Nine extraction methods (three using chlorinated and six nonchlorinated solvents) were compared for determining lipids in samples of low‐(<5%) and high‐fat (>20%) ground beef. The nine methods investigated were: Folch, Lees, and Sloane Stanley (FLS); Bligh and Dyer (BD); Bligh and Dyer modifie...

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Bibliographic Details
Published inJournal of the American Oil Chemists' Society Vol. 82; no. 6; pp. 393 - 397
Main Authors Tanamati, A, Oliveira, C.C, Visentainer, J.V, Matsushita, M, Souza, N.E. de
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer‐Verlag 01.06.2005
Springer
Springer Nature B.V
Subjects
Beef
Biological and medical sciences
chemical analysis
chlorides
chlorinated solvents
Chloroform
Comparative studies
extraction
extraction methods
fat
Fat industries
Food engineering
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
ground beef
Hara
lipid composition
lipid content
Lipids
meat composition
meat extracts
methanol
Organic solvents
Solvent extraction processes
Solvents
total lipids
Oils and fats industry
Toxicity
Meat product
Fat
Lipids
Extraction
total lipids
Method
Beef
chlorinated solvents
Solvent
extraction methods
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ISSN0003-021X
1558-9331
DOI10.1007/s11746-005-1083-4

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Summary:Nine extraction methods (three using chlorinated and six nonchlorinated solvents) were compared for determining lipids in samples of low‐(<5%) and high‐fat (>20%) ground beef. The nine methods investigated were: Folch, Lees, and Sloane Stanley (FLS); Bligh and Dyer (BD); Bligh and Dyer modified by Undeland, Harrod, and Lingnert (BDU); Bligh and Dyer modified by Smedes (BDS); Hara and Radin (HR); Schmid, Bondzynski, and Ratzalaff (SBR); Roese‐Gottlieb (RG); Burton, Webb, and Ingold (BWI); and Soxhlet (SE). The BDS and HR methods do not include solvents such as chloroform and methanol and can be recommended for meat samples that have both low and high fat content. If the use of organic solvents is not critical, the FLS and the BD extraction methods yield the highest results, but the FLS is more expensive owing to the high amount of solvent required. Without considering the toxicity of the solvents, the three BD extraction methods provided the best yields.
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ISSN:0003-021X
1558-9331
DOI:10.1007/s11746-005-1083-4