Quantum measurement enables single biomarker sensitivity in flow cytometry
We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual bi...
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| Published in | Scientific reports Vol. 14; no. 1; pp. 3891 - 8 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
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London
Nature Publishing Group UK
16.02.2024
Nature Publishing Group Nature Portfolio |
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| ISSN | 2045-2322 2045-2322 |
| DOI | 10.1038/s41598-023-49145-7 |
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| Abstract | We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual biomarkers traversing the interrogation volume of the flow cytometer from the first principles. This observation enables the use of the quantum toolbox for rapid detection, enumeration, and sorting of single fluorophores in large cell populations as well as a ‘photons-to-moles’ calibration of this measurement modality. |
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| AbstractList | We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual biomarkers traversing the interrogation volume of the flow cytometer from the first principles. This observation enables the use of the quantum toolbox for rapid detection, enumeration, and sorting of single fluorophores in large cell populations as well as a 'photons-to-moles' calibration of this measurement modality. Abstract We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual biomarkers traversing the interrogation volume of the flow cytometer from the first principles. This observation enables the use of the quantum toolbox for rapid detection, enumeration, and sorting of single fluorophores in large cell populations as well as a ‘photons-to-moles’ calibration of this measurement modality. We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual biomarkers traversing the interrogation volume of the flow cytometer from the first principles. This observation enables the use of the quantum toolbox for rapid detection, enumeration, and sorting of single fluorophores in large cell populations as well as a 'photons-to-moles' calibration of this measurement modality.We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual biomarkers traversing the interrogation volume of the flow cytometer from the first principles. This observation enables the use of the quantum toolbox for rapid detection, enumeration, and sorting of single fluorophores in large cell populations as well as a 'photons-to-moles' calibration of this measurement modality. |
| ArticleNumber | 3891 |
| Author | Burenkov, I. A. Polyakov, S. V. Sabines-Chesterking, J. |
| Author_xml | – sequence: 1 givenname: J. surname: Sabines-Chesterking fullname: Sabines-Chesterking, J. organization: Joint Quantum Institute, University of Maryland, National Institute of Standards and Technology – sequence: 2 givenname: I. A. surname: Burenkov fullname: Burenkov, I. A. organization: Joint Quantum Institute, University of Maryland, National Institute of Standards and Technology – sequence: 3 givenname: S. V. surname: Polyakov fullname: Polyakov, S. V. email: sergey.polyakov@nist.gov organization: National Institute of Standards and Technology, Department of Physics, University of Maryland |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38365797$$D View this record in MEDLINE/PubMed |
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| Title | Quantum measurement enables single biomarker sensitivity in flow cytometry |
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