Quantum measurement enables single biomarker sensitivity in flow cytometry

We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual bi...

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Bibliographic Details
Published inScientific reports Vol. 14; no. 1; pp. 3891 - 8
Main Authors Sabines-Chesterking, J., Burenkov, I. A., Polyakov, S. V.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 16.02.2024
Nature Publishing Group
Nature Portfolio
Subjects
631/1647/1407/1492
639/624/1075/1083
639/766/400/482
639/766/483/3925
Aperture
Biomarkers
Calibration
Enumeration
Experimental methods
Flow cytometry
Flow Cytometry - methods
Fluorescent Dyes
Fluorophores
Humanities and Social Sciences
Lasers
Light
multidisciplinary
Nanowires
Photons
Quantum dots
Questioning
Science
Science (multidisciplinary)
Sensors
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ISSN2045-2322
2045-2322
DOI10.1038/s41598-023-49145-7

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Summary:We present the first unambiguous experimental method enabling single-fluorophore sensitivity in a flow cytometer using quantum properties of single-photon emitters. We use a quantum measurement based on the second-order coherence function to prove that the optical signal is produced by individual biomarkers traversing the interrogation volume of the flow cytometer from the first principles. This observation enables the use of the quantum toolbox for rapid detection, enumeration, and sorting of single fluorophores in large cell populations as well as a ‘photons-to-moles’ calibration of this measurement modality.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-49145-7