The interaction between methanol dehydrogenase and the autoreducible cytochromes c of the facultative methylotroph Pseudomonas AM1

Cytochromes cH and cL were autoreduced at high pH (pK greater than 10) and the autoreduced cytochromes reacted with CO. The autoreduction was first-order with respect to oxidized cytochrome c and was reversible by lowering the pH. Pure methanol dehydrogenase reduced cytochrome c (in the absence of m...

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Bibliographic Details
Published inBiochemical journal Vol. 190; no. 2; pp. 481 - 484
Main Authors O'Keeffe, D T, Anthony, C
Format Journal Article
LanguageEnglish
Published England 15.08.1980
Subjects
Alcohol Oxidoreductases - metabolism
Cytochrome c Group - metabolism
Kinetics
Methanol
Models, Biological
Oxidation-Reduction
Pseudomonas - enzymology
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ISSN0306-3283
0264-6021
1470-8728
DOI10.1042/bj1900481

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Summary:Cytochromes cH and cL were autoreduced at high pH (pK greater than 10) and the autoreduced cytochromes reacted with CO. The autoreduction was first-order with respect to oxidized cytochrome c and was reversible by lowering the pH. Pure methanol dehydrogenase reduced cytochrome c (in the absence of methanol) by lowering the pK for autoreduction to less than 8.5. A mechanism is proposed for the autoreduction of cytochrome c and its involvement in the reaction with methanol dehydrogenase.
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ISSN:0306-3283
0264-6021
1470-8728
DOI:10.1042/bj1900481