Divergent Immunomodulatory Effects of Extracts and Phenolic Compounds from the Fern Osmunda Japonica Thunb

• Published in: Chinese journal of integrative medicine Vol. 19; no. 10; pp. 761 - 770
• Main Author: 朱晓新 李玉洁 杨岚 张东 陈颖 EvaKmonickova 翁小刚 杨庆 Zdenek Zidek
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2013
• Subjects: Animals; Cell Survival - drug effects; Cells, Cultured; Dinoprostone - biosynthesis; Female; Ferns - chemistry; Immunologic Factors - pharmacology; Interferon-gamma - pharmacology; Lipopolysaccharides - pharmacology; Medicine; Medicine & Public Health; Nitric Oxide - biosynthesis; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Original Article; Peritoneum - cytology; Peritoneum - drug effects; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Polymyxin B - pharmacology; Proline - analogs & derivatives; Proline - pharmacology; Rats; Rats, Wistar; RNA, Messenger - genetics; RNA, Messenger - metabolism; Thiocarbamates - pharmacology; TNF-α; 乙醇提取物; 免疫调节作用; 白细胞介素-1; 羟基苯甲醛; 薇菜; 酚类化合物; 酶联免疫吸附试验; phenolic compounds; nitric oxide; extracts; cytokines; L; prostaglandin E
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-013-1460-4

Objective： To study possible immunobiological potential of Osmunda japonica Thunb. Methods： Immunomodulatory effects of ethanol extracts prepared from rhizomes of O. japonica and phenolic compounds isolated from the extracts were investigated under the in vitro conditions using the rat peritoneal cells （2 × 10_6/mL; 24 h culture）. Biosynthesis of nitric oxide （NO） was assayed by Griess reagent, production of prostaglandin E2 （PGE2） and secretion of cytokines were determined by enzyme-linked immunoabsorbent assay. Resalts： The extracts activated dose dependently, with the onset at 2.5-5 μ mol/L concentrations, the high output NO production, and secretion of interferon-r, （IFN-r）, tumor necrosis factor-oL （TNF-α） and interleukin-1 （IL-1 13 ）. Mild enhancement of NO was produced by the aldehyde-type phenolics 4-hydroxybenzaldehyde and 3,4-hydroxybenzaldehyde. In contrasts, the acetone-type phenolics 4-hydroxybenzalacetone and 3,4-hydroxybenzalacetone inhibited production of immune mediators including cytokines （TNF-α, IL-β, IL-6）, NO, and PGE2. The 3,4-hydroxybenzalacetone was more effective than 4-hydroxybenzaldehyde. The ICsoS estimates ranged within the interval of 5-10 μ mol/L. No signs of cytotoxicity were observed up to the 50 μ-mol/L concentration of the compounds. Coaclasiea： Phenolic compounds contained in medicinal herb Osmunda japonica possess distinct immunomodulatory activity.