Production of Bio-Based Isoprene by the Mevalonate Pathway Cassette in Ralstonia eutropha

• Published in: Journal of microbiology and biotechnology Vol. 29; no. 10; pp. 1656 - 1664
• Main Authors: Lee, Hyeok-Won, Park, Jung-Ho, Lee, Hee-Seok, Choi, Wonho, Seo, Sung-Hwa, Anggraini, Irika Devi, Choi, Eui-Sung, Lee, Hong-Weon
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.10.2019
• Subjects: Bacterial Proteins - genetics; Biosynthetic Pathways - genetics; Butadienes; Cupriavidus necator - genetics; Cupriavidus necator - metabolism; Escherichia coli - genetics; Fermentation; Hemiterpenes - biosynthesis; Metabolic Engineering; Mevalonic Acid - metabolism; Promoter Regions, Genetic; 생물학; fermentation; Ralstonia eutropha; promoter; isoprene production; MVA pathway
• ISSN: 1017-7825; 1738-8872; 1738-8872
• DOI: 10.4014/jmb.1909.09002

Isoprene has the potential to replace some petroleum-based chemicals and can be produced through biological systems using renewable carbon sources. can produce value-added compounds, including intracellular polyhydroxyalkanoate (PHA) through fatty acid and lipid metabolism. In the present study, we engineered strains of H16 and examined the strains for isoprene production. We optimized codons of all the genes involved in isoprene synthesis by the mevalonate pathway and manipulated the promoter regions using pLac and pJ5 elements. Our results showed that isoprene productivity was higher using the J5 promoter (1.9 ± 0.24 µg/l) than when using the lac promoter (1.5 ± 0.2 µg/l). Additionally, the use of three J5 promoters was more efficient (3.8 ± 0.18 µg/l) for isoprene production than a one-promoter system, and could be scaled up to a 5-L batch-cultivation from a T-flask culture. Although the isoprene yield obtained in our study was insufficient to meet industrial demands, our study, for the first time, shows that can be modified for efficient isoprene production and lays the foundation for further optimization of the fermentation process.