Levan production from sucrose using chicken feather peptone as a low cost supplemental nutrient source

• Published in: Carbohydrate polymers Vol. 227; p. 115361
• Main Authors: Veerapandian, Bhuvaneshwari, Shanmugam, Saravanan Ramiah, Varadhan, Srivathsan, Sarwareddy, Kartik Kumar, Mani, Krishna Priya, Ponnusami, V.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2020
• Subjects: Acid hydrolysis; amino acids; Animals; Bacillus subtilis; Bacillus subtilis - metabolism; carbon; cell lines; Cell Survival; Chicken feather peptone; Chicken feathers; Chickens; dietary minerals; Feathers - chemistry; Fermentation; Fructans - metabolism; Fructans - pharmacology; Human Umbilical Vein Endothelial Cells - drug effects; Humans; levan; Levan production; peptones; Peptones - metabolism; poultry manure; sucrose; Sucrose - metabolism; Levan production; Acid hydrolysis; Chicken feathers; Chicken feather peptone
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2019.115361

[Display omitted] •Chicken feather peptone (CFP) from poultry waste was synthesized.•CFP was utilized as a low cost nutrient source for microbial biopolymer production.•Levan produced from CFP was found to be non-toxic and biocompatible in cell lines.•CFP could be utilized as a low-cost nutrient source in fermentation industry. Chicken feather peptone (CFP) derived from poultry waste is a rich source of essential minerals and amino acids. This, along with suitable carbon source, can be used as a low cost complex supplemental nutrient source for microbial fermentation. In the present work, CFP blended with sucrose was evaluated for the production of levan using Bacillus subtilis MTCC 441. Amount of CFP added to the medium significantly influenced levan production and it was found that at a concentration 2 g/L, maximum levan yield of 0.26 ± 0.04 g/g sucrose was obtained. The levan yield obtained with CFP as a low cost supplemental nutrient source was comparable with that obtained from commercial medium (0.31 ± 0.02 g/g sucrose). Levan produced using CFP was tested on primary cell lines at various concentrations (100–1000 μM) and found to be non-toxic and bio-compatible in nature. This indicates that CFP could be used as low cost nutrient source for levan production.