The impact of anticancer activity upon Beta vulgaris extract mediated biosynthesized silver nanoparticles (ag-NPs) against human breast (MCF-7), lung (A549) and pharynx (Hep-2) cancer cell lines

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 173; pp. 99 - 107
• Main Authors: Venugopal, K., Ahmad, H., Manikandan, E., Thanigai Arul, K., Kavitha, K., Moodley, M.K., Rajagopal, K., Balabhaskar, R., Bhaskar, M.
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01.08.2017; Elsevier BV
• Subjects: A549 Cells; Anticancer activity; Anticancer properties; antineoplastic activity; antineoplastic agents; Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antitumor activity; Antitumor agents; Apoptosis; Apoptosis - drug effects; Beta vulgaris; Beta vulgaris - chemistry; Beta vulgaris - metabolism; Biomarkers; Biotechnology; breast neoplasms; C band; Cancer; Cell death; Cell Line, Tumor; Cytotoxicity; Fabrication; Fourier transforms; FTIR technique; Gold; Green Chemistry Technology; Green synthesis; growth retardation; human cell lines; Humans; In vivo methods and tests; lung neoplasms; Lungs; MCF-7 Cells; Metal Nanoparticles - chemistry; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Nanoparticles; nanosilver; neoplasm cells; Particle Size; Pharynx; Plant extracts; Plant Extracts - chemistry; Silver; Silver - chemistry; Silver nanoparticles; Spectrophotometry, Ultraviolet; Spectroscopy; Spectroscopy, Fourier Transform Infrared; staining; TEM + EDAX; temperature; transmission electron microscopy; Tumor cell lines; Ultraviolet radiation; ultraviolet-visible spectroscopy; UV–visible; Variation; TEM+EDAX; Green synthesis; UV–visible; FTIR technique; Silver nanoparticles; Anticancer activity
• ISSN: 1011-1344; 1873-2682; 1873-2682
• DOI: 10.1016/j.jphotobiol.2017.05.031

The present study tried for a phyto-synthetic method of producing silver nanoparticles (Ag-NPs) with size controlled as and eco-friendly route that can lead to their advanced production with decorative tranquil morphology. By inducing temperature fluctuation of the reaction mixture from 25 to 80°C the plasmon resonance band raised slowly which had an ultimate effect on size and shape of Ag-NPs as shown by UV–visible spectroscopy and TEM results. The biosynthesized nanoparticles showed good cytotoxic impact against MCF-7, A549 and Hep2 cells compared to normal cell lines. Compared to control plates, the percentage of cell growth inhibition was found to be high with as concentrations of Ag-NPs becomes more as determined by MTT assay. The AO/EtBr staining observations demonstrated that the mechanism of cell death induced by Ag-NPs was due to apoptosis in cancer cells. These present results propose that the silver nanoparticles (Ag-NPs) may be utilized as anticancer agents for the treatment of various cancer types. However, there is a need for study of in vivo examination of these nanoparticles to find their role and mechanism inside human body. Further, studies we plan to do biomarker fabrication from the green synthesized plant extract nanoparticles like silver, gold and copper nanoparticles with optimized shape and sizes and their enhancement of these noble nanoparticles. [Display omitted] •Phytosynthetic (Beta vulgaris) method of producing silver nanoparticles (AgNPs) as an eco-friendly•The SPR peaks of Ag-NPs for different temperatures shown at ~445 to 465nm•Ag-NPs HRTEM depicts spherical, circular & triangular shapes with particles size 5–20nm.•Biosynthesized Ag-NPs showed toxicity towards cancer cell line while no impact on normal cells.