UVB and γ-Radiation Induce the Expression of mRNAs Encoding the Ribosomal Subunit L13A in Rat Keratinocytes

• Published in: Photochemistry and photobiology Vol. 70; no. 3; pp. 341 - 347
• Main Authors: Shahmolky, Nazanin, Lefebvre, Diana L., Poon, Raymond, Bai, Yahong, Sharma, Monika, Rosen, Cheryl F.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.1999
• Subjects: Animal tissues; Animals; Cells, Cultured; Deoxyribonucleic acid; DNA; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Gamma Rays; Gene Expression Regulation - radiation effects; Irradiation; Keratinocytes - metabolism; Keratinocytes - radiation effects; Rats; Ribosomal Proteins - biosynthesis; Ribosomal Proteins - genetics; RNA, Messenger - biosynthesis; RNA, Messenger - isolation & purification; Ultraviolet Rays
• ISSN: 0031-8655; 1751-1097
• DOI: 10.1111/j.1751-1097.1999.tb08146.x

Ultraviolet B radiation produces an array of cellular perturbations in the skin. We isolated a keratinocyte cDNA encoding the rat 60S ribosomal subunit protein L13a following differential cDNA library screening with UVB‐en‐riched probes. In contrast to the reported structure of liver L13a, the keratinocyte L13a cDNA contains a longer 3′‐untranslated region. Northern blot analysis detected two L13a mRNA transcripts, ?800 bp and ?1.2 kb, in keratinocytes and a variety of rat tissues. Both L13a mRNA transcripts were induced by UVB irradiation, for‐skolin and ‐γ‐irradiation. In contrast, no induction of L13a mRNA transcript levels was observed following exposure of keratinocytes to 12‐O‐tetradecanoylphorbol‐13‐acetate, serum and the DNA damage‐inducing agents methyl methanesulfonate or 4‐nitroquinoUne‐N‐oxide. These observations suggest that increased expression of ribosomal subunit genes may be a molecular component of the keratinocyte response to UVB in particular and not part of a nonspecific response to DNA damage.