Matrix Metallopeptidase 2 Gene Polymorphism is Associated with Obesity in Korean Population

• Published in: The Korean journal of physiology & pharmacology Vol. 12; no. 3; pp. 125 - 129
• Main Authors: Han, Dong Hee, Kim, Su Kang, Kang, Sungwook, Choe, Bong-Keun, Kim, Keon Sik, Chung, Joo-Ho
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Physiological Society and The Korean Society of Pharmacology 01.06.2008; 대한약리학회
• Subjects: Original; 약리학; Matrix metallopeptidase 2; Body mass index; Obesity; Single nucleotide polymorphism; Linkage disequilibrium
• ISSN: 1226-4512; 2093-3827; 2093-3827
• DOI: 10.4196/kjpp.2008.12.3.125

The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of matrix metallopeptidase 2 (MMP2) are associated with obesity. MMP2 is an enzyme with proteolytic activity against matrix and nonmatrix proteins, particularly basement membrane constituents. To identify the relationship between polymorphisms of MMP2 and overweight/obese, we genotyped 5 SNPs (rs17242319, rs1053605, rs243849, rs2287074, and rs10775332) of the coding region of MMP2 using the Golden Gate assay on an Illumina BeadStation 500 GX. One hundred and forty two overweight/obese (BMI >/=23) and 145 normal (BMI 18 to <23) subjects were analyzed. SNPStats, Haploview, HapAnalyzer, SNPAnalyzer, and Helixtree programs were used for the analysis of genetic data. A linkage disequilibrium (LD) block was discovered among the 5 SNPs selected, including rs17242319, rs1053605, rs243849, and rs2287074. Of the 5 polymorphisms, 2 synonymous SNPs [rs17242319 (Gly226Gly) and rs10775332 (Phe602Phe)] were found significant associations with overweight/obese. Recently, rs1132896 replaced rs17242319 as a new number (SNP database, BUILD 129). In haplotype analysis using Haploview, a haplotype (haplotype: CCCA) containing a meaningful polymorphism (rs17242319) was found to be significantly different. The results suggest that MMP2 may be associated with overweight/obese in Korean population.