Hsa-circRNA11783-2 in peripheral blood is correlated with coronary artery disease and type 2 diabetes mellitus

• Published in: Diabetes & vascular disease research Vol. 14; no. 6; pp. 510 - 515
• Main Authors: Li, Xuejie, Zhao, Zhenzhou, Jian, Dongdong, Li, Wentao, Tang, Haiyu, Li, Muwei
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.11.2017
• Subjects: Case-Control Studies; Cell-Free Nucleic Acids - blood; Cell-Free Nucleic Acids - genetics; Chi-Square Distribution; Coronary Artery Disease - blood; Coronary Artery Disease - diagnosis; Coronary Artery Disease - genetics; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - diagnosis; Diabetes Mellitus, Type 2 - genetics; Down-Regulation; Gene Expression Profiling - methods; Genetic Markers; Humans; Logistic Models; Odds Ratio; Oligonucleotide Array Sequence Analysis; Original; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA - blood; RNA - genetics; type 2 diabetes mellitus; microarray analysis; circulating circular RNA; Coronary artery disease; circular RNA
• ISSN: 1479-1641; 1752-8984; 1752-8984
• DOI: 10.1177/1479164117722714

Objective: The purpose of this study was to identify the expression characteristics of circular RNAs in the peripheral blood of coronary artery disease patients and type 2 diabetes mellitus patients. Methods: Circular RNA in the peripheral blood from 6 control individuals, 6 coronary artery disease patients, 6 type 2 diabetes mellitus patients and 6 coronary artery disease combined with type 2 diabetes mellitus patients was collected for microarray analysis, and a further independent cohort consisting of 20 normal individuals, 20 type 2 diabetes mellitus subjects and 20 coronary artery disease subjects was used to verify the expression of five circular RNAs chosen for further analysis. The findings were then tested in a third cohort using quantitative real-time polymerase chain reaction. Results: In total, 40 circular RNAs differentially expressed between the three experimental groups and the control group were identified by microarray analysis: 13 were upregulated in the experimental groups, while 27 were downregulated. Of the five circular RNAs chosen for further analysis, three were significantly downregulated in the experimental groups. The crude odds ratios and adjusted odds ratios of hsa-circRNA11783-2 showed significant differences in both the coronary artery disease group and type 2 diabetes mellitus group. We then verified hsa-circRNA11783-2 in the third cohort, and it remained closely related to both coronary artery disease and type 2 diabetes mellitus. Conclusion: Hsa-circRNA11783-2 is closely related to both coronary artery disease and type 2 diabetes mellitus.