Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus

• Published in: Chinese journal of oceanology and limnology Vol. 26; no. 1; pp. 62 - 68
• Main Author: 罗文 赵云龙 周忠良 安传光 马强
• Format: Journal Article
• Language: English
• Published: Beijing Science Press 01.02.2008; Springer Nature B.V; School of Life Science,College of Shaoxing Arts and Sciences,Shaoxing 312000,China; School of Life Science,East China Normal University,Shanghai 200062,China%School of Life Science,East China Normal University,Shanghai 200062,China
• Subjects: Animal embryos; Antigens; Aquaculture; Biochemistry; Cambaridae; Cherax; Cherax quadricarinatus; Chymotrypsin; Crayfish; Crustaceans; Developmental stages; Earth and Environmental Science; Earth Sciences; Embryogenesis; Embryonic development; Embryonic growth stage; Enzymatic activity; Enzyme activity; Enzymes; Freshwater; Freshwater crustaceans; Gene expression; Hatching; Larvae; Marine biology; Nucleotide sequence; Oceanography; Proteins; Regulatory mechanisms (biology); Ribonucleic acid; RNA; Trypsin; Trypsin gene; 消化酶活性; 胰凝乳蛋白酶; 胰岛素; trypsin; mRNA; chymotrypsin; protein; Cherax quadricarinatus
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-008-0062-z

Abstract The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR （FQ-PCR） methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However, chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity, but also the regulatory mechanism for expression of trypsin gene to a certain degree.